Assessment of host biochemical factors and microbiota interactions and pathogenicity of Blastocystis hominis genotypes: A cross-sectional study | ||
| Parasitologists United Journal | ||
| Article 4, Volume 16, Issue 3, December 2023, Pages 183-191 PDF (541.84 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/puj.2023.239538.1224 | ||
| Authors | ||
| Enas El Saftawy1; Marwa Ghallab2; Tamer Haydara3; Asmaa Ibrahim4; Shimaa Atta5; Reham Gad Allah5; Marwa Kamel6; Salwa Morsy* 7 | ||
| 1Departments of Medical Parasitology, Faculty of Medicine,Cairo University, Armed Forces College of Medicine,Egypt | ||
| 2Departments of Medical Parasitology, Faculty of Medicine,Kafr El-Sheikh University,Egypt | ||
| 3Internal Medicine,Kafr El-Sheikh University,Egypt | ||
| 4Biotechnology Research Institute, Sadat City University5,Egypt | ||
| 5Theodor Bilharz Research Institute,Egypt | ||
| 6Master in Quality Management, Arab Academy for Science, Technology, and Maritime Transport, Productivity and Quality Institute, Smart Village | ||
| 7Departments of Medical Parasitology, Faculty of Medicine , Cairo University& Modern University for Technology and Information , Egypt | ||
| Abstract | ||
| Background: Although several clinical and scientific evidences confirmed B. hominis pathogenicity in irritable bowel syndrome (IBS), host biochemical risk factors involved in IBS manifestations are still ambiguous. Objective: To investigate the interactions between B. hominis genotypes, host biochemical factors, and microbiota. Patients and Methods: In a cross-sectional study, 167 stool samples from patients attending the Internal Medicine Department, of Kafr El-Sheikh University Hospital were examined. Using specific sequencedtagged site (STS) primers, samples positive for B. hominis were subtyped. Reducing sugar and pH were assessed in B. hominis-positive samples. The lactate dehydrogenase (LDH) enzyme was measured in both B. hominis sole infections and blastocystosis coexisting with G. intestinalis. To determine the predominant co-existing organisms in blastocystosis infections, bacterial and fungal stool cultures were performed. Furthermore, laboratory-cultivated B. hominis was incubated in vitro with Escherichia (Esch.) coli and Candida non-albicans to assess possible interactions with models of normal microbiota. Results: Of 167 cases, thirty-three (19.7%) were microscopically positive, and twenty seven (16.2%) were molecularly confirmed harboring B. hominis. Genotype 3 was solely detected with higher prevalence in summer and spring. There was a consistent chemical association of carbohydrate intolerance and acidic pH with genotype-3 B. hominis that seemed to augment IBS-like manifestations. Predominant overgrowth of Esch. coli in cultured stool samples was observed. Co-culture of B. hominis with Esch. coli and C. nonalbicans augmented their growth whereas the parasite was suppressed. Coinfection of B. hominis with G. intestinalis showed a significant rise in LDH enzyme in stool samples compared with the presence of B. hominis alone. Conclusion: Genotype-3 B. hominis is predominant and closely related to IBS-like manifestations. Blastocystosis appeared to be related to carbohydrate intolerance, fecal acidity, and enhanced effect on the growth of Esch. coli and C. non-albicans. | ||
| Keywords | ||
| B. hominis; Candida; Escherichia coli; genotype 3; lactate dehydrogenase; microbiota; reducing sugar | ||
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