PREPARATION AND EVALUATION OF AN INACTIVATED COMBINED VACCINE OF BOVINE ROTA, CORONA VIRUSES AND K99 ENTEROTOXIGENIC ESCHERICHIA COL! | ||
| Egyptian Journal of Agricultural Research | ||
| Article 34, Volume 77, Issue 2, July 1999, Pages 957-969 PDF (2.53 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejar.1999.336283 | ||
| Authors | ||
| MOHAMED S. WASSEL; SAMY M. SHAFEY; SALEH S. MOHAMED | ||
| Central Laboratory for control of Veterinary Biologics, Agricultural Research Centre, Giza, Egypt | ||
| Abstract | ||
| A formalin inactivated alum gel adjuvated combined vaccine of bovine rotavirus (BRV), bovine coronavirus (BCV) and K99 enterotoxigenic Escherichia coil (ETEC) was prepared and evaluated. Ten susceptible calves were vaccinated intramuscularly twice 2 weeks apart using 2 ml/calf. The vaccine was tested for safety in calves and mice and for sterility. Sera of immunized calves showed high antibody levels (4-8 log2 for both BRV and BCV) using serum neutralization test. The ELISA antibody titres were also satisfactory high for the three vaccine components. Passive mouse protection test revealed 80-100% protection against K99 ETEC component. The microagglutination test was also used for evaluation of the potency of K99 ETEC and the titres obtained reached 320 and 1810 at 2 and 8 weeks postvaccination, respectively. It is recommended to prepare the trivalent vaccine locally for control of diarrhoea in calves. | ||
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