The influence of lncRNA expression dysregulation on predicting celiac diseases among patients with Hashimoto's thyroiditis | ||
| African Journal of Gastroenterology and Hepatology | ||
| Volume 7, Issue 1, 2024, Pages 110-128 PDF (653.51 K) | ||
| Document Type: Original Clinical | ||
| DOI: 10.21608/ajgh.2024.262342.1048 | ||
| Authors | ||
| Nearmeen M. Rashad* 1; Mohamed Othman Wahba1; Abdelmonem Mohamed Elshamy2; Manar H. Soliman3; Marwa H.S Hussien4; Ahmad Sallam5; Amr Talaat EL Hawary1 | ||
| 1Internal Medicine Department, Faculty of Medicine, Zagazig University, Egypt. | ||
| 2Tropical Medicine department, Faculty of Medicine, Zagazig University, Egypt. | ||
| 3Medical Microbiology and Immunology Department, faculty of medicine, Zagazig University, Egypt. | ||
| 4Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Egypt. | ||
| 5Clinical Pathology Department, Faculty of Medicine, Zagazig University, Egypt. | ||
| Abstract | ||
| Aim: the study aimed to investigate lncRNA IFNG-AS1 in Hashimoto’s thyroiditis (HT) and to evaluate its predictive and diagnostic values of Celiac disease (CD) among patients with HT. Patients and Methods One hundred voluntary subjects: 50 healthy controls and 50 patients with HT;39 without CD and 11 with CD. Routine labs, including anti-TG, anti-TPO, and anti-TTG-IgA, investigated all subjects. lncRNA IFNG-AS1 expression was analyzed using Quantitative real-time RT-PCR. Results Patients with CD had significantly overexpressed lncRNA IFNG-AS1(4.17±0.618) compared to patients without CD (1.92±0.52) and control group (0.98±0.21), p ˂0.001*. It correlated considerably with intestinal and extra-intestinal manifestations and laboratory tests, including anti-TG, anti-TPO, and TTG IgA. Multivariate regression confirmed that the only variables independently associated with lncRNA IFNG-AS1 in the prediction of CD among studied parameters were anti-TG, anti-TPO, and TTG IgA. We found high sensitivity and specificity of 96% and 94% among HT, respectively. Concerning CD, the predictive power of IFNG-AS1 had sensitivity and specificity of 63.3% and 53%, respectively. Conclusion: lncRNA IFNG-AS1 level was overexpressed in patients with HT, particularly those with CD, and correlated to clinical and laboratory parameters. Accordingly, it may be a promising predictive biomarker for HT and CD. | ||
| Keywords | ||
| anti-TPO; anti-TG; lncRNAs; CD; TTG IgA; HT; IFNG-AS1; RT-PCR; gluten intolerance; enteropathy | ||
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