Combined sperm selection techniques to boost the success of in Vitro Fertilization (IVF) and Intra Cytoplasmic Sperm Injection (ICSI) | ||
| Research Journal of Applied Biotechnology | ||
| Article 2, Volume 9, Issue 2, December 2023, Pages 13-23 PDF (413.47 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/rjab.2023.233720.1042 | ||
| Authors | ||
| Dina Ibrahem Abd El-baseer* 1; bahgat A Elifky2; Khaled, A Salem3; Ebrahim Abdul mohsen Sabra4 | ||
| 1Animal biotechnology department - Genetic Engineering and Biotechnology Research Institute | ||
| 2Animal biotechnology department, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City, Egypt. | ||
| 3Urology Department, Faculty of Medicine, Tanta University, Egypt. | ||
| 4Animal biotechnology department,genetic engineering and biotechnology research institute , university of Sadat city,Egypt | ||
| Abstract | ||
| This study aimed to assess the effectiveness of combined methods of Density gradient centrifugation and swim-up (D.G.C/S. U) and Density gradient centrifugation and zeta potential (D.G.C/Z) in semen samples to treat sperm abnormality, decrease DNA fragmentation, and choose one of the best methods for Teratozoospermia patients who have high Sperm DNA Fragmentation (SDF) undergoing assisted reproduction. Method: 101 patients, who all have teratozoospermia with high DNA fragmentation visited the fertility clinic. Semen features were examined using established criteria According to Recent World Health Organization (WHO), DGC /Z and DGC/ SU methods were carried out on semen samples After that, the samples were evaluated by Sperm Chromatin Dispersion (SCD) testing was used by the WHO to detect DNA damage recently. Result:The first combined method used showed statistically significant higher motility with DGC/su(p < 0.001), while the other method showed statistically significant, lower DNA fragmentation and fewer abnormalities was DGC/Z(p < 0.001). We discovered no statistically significant connection between defective sperm morphology and DNA damage. There is no link between sperm motility and DNA damage, indicating that defective sperm are more likely to be normal DNA. Conclusion: According to the results of the current study, the combined use of the DGC/Z and DGC/swim-up procedures improved the motility, morphology, and DNA integrity of semen samples, which may increase the probability of a successful pregnancy. | ||
| Keywords | ||
| DNA Fragmentation; Sperm selection; Zeta method. Swim-up. Density gradient | ||
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