Effect of Apitoxin on Alveolar Bone Alterations in Induced Diabetic Rats (An Animal Study) | ||
| Egyptian Journal of Histology | ||
| Article 23, Volume 46, Issue 4, December 2023, Pages 1870-1879 PDF (662.04 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejh.2022.146295.1707 | ||
| Authors | ||
| iman aboushady* 1; dina badawy farag2 | ||
| 1oral biology department, faculty of dentistry, Cairo university, Egypt | ||
| 2Oral Biology Department, Faculty of Dentistry, Cairo University | ||
| Abstract | ||
| Introduction: Diabetes mellitus (DM) is a metabolic disorder caused by deficient insulin secretion or action or both. Among the main complications of DM is altered bone metabolism. Apitoxin, or honey bee venom (BV), has recently been suggested to have beneficial therapeutic effects against various diseases, including diabetes. Objective: Evaluation of the possible therapeutic effect of BV in ameliorating diabetes-induced alterations in rat’s alveolar bone. Materials and Methods: 18 adult male rats, about 3-4 months age, were utilized. 6 rats served as control (Group I), DM was induced in the remaining rats through streptozotocin injection. Diabetic rats were randomly divided into: Group II (treated with vehicle), Group III (treated with BV). All rats were sacrificed after 1 month, mandibles were examined histologically, by scanning electron microscopy and by RT-PCR to assess osteoprotegerin (OPG) and interleukin (IL)-17 genes’ expression. Results: Histologically, group I showed a normal alveolar bone structure, while following diabetes induction, in group II, degenerative changes were noticed. Treatment with BV (group III) resulted in a relatively well restored histological structure of alveolar bone. Scanning electron microscopic results revealed a smooth buccal cortical plate in group I, while in group II, a generalized destruction was noticed. However; in group III, the bone surface demonstrated slight roughness. Statistical results presented a significant decrease in OPG gene expression in both group II and III than in group I. However; OPG gene expression was significantly increased in group III in comparison to group II. A significant increase occurred also in IL-17 gene expression in groups II and III. On the other hand, this expression significantly decreased in group III than in group II. Conclusion: These results could suggest that BV treatment could ameliorate diabetes-induced alveolar bone changes; this could be attributed to the anti-osteoclastogenic and anti-inflammatory effects of BV. | ||
| Keywords | ||
| Apitoxin; bee venom; diabetic rats; IL-17; osteoprotegerin | ||
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