Soliman, A., Awad, A., selim, A. (2023). Synthesis and characterization of garlic oil nanoemulsion to detect its antibacterial effect against Trueperella pyogenes. test, 45(2), 126-131. doi: 10.21608/bvmj.2023.237160.1727
Aya Medhat Soliman; Ashraf Awad; Amany omar selim. "Synthesis and characterization of garlic oil nanoemulsion to detect its antibacterial effect against Trueperella pyogenes". test, 45, 2, 2023, 126-131. doi: 10.21608/bvmj.2023.237160.1727
Soliman, A., Awad, A., selim, A. (2023). 'Synthesis and characterization of garlic oil nanoemulsion to detect its antibacterial effect against Trueperella pyogenes', test, 45(2), pp. 126-131. doi: 10.21608/bvmj.2023.237160.1727
Soliman, A., Awad, A., selim, A. Synthesis and characterization of garlic oil nanoemulsion to detect its antibacterial effect against Trueperella pyogenes. test, 2023; 45(2): 126-131. doi: 10.21608/bvmj.2023.237160.1727

Synthesis and characterization of garlic oil nanoemulsion to detect its antibacterial effect against Trueperella pyogenes

Benha Veterinary Medical Journal
Volume 45, Issue 2, December 2023, Pages 126-131    PDF (375.64 K)
Document Type: Original Article
DOI: 10.21608/bvmj.2023.237160.1727
Authors
Aya Medhat Soliman* 1; Ashraf Awad1; Amany omar selim2
1Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Benha University, Kalyobiya, Egypt
2Bacteriology Department, Benha Provincial Laboratory, Animal Health Research Institute, Agriculture Research Center, Egypt
Abstract
On account of the pivotal role of herbal compounds in all scientific research, this study aimed to detect the effect of garlic essential oil nanoemulsion (GON) on Trueperella pyogenes (T. pyogenes). Four isolates of T. pyogenes were grown on brain heart infusion agar were used for extraction of the bacterial DNA for detection of 16 SrRNA and rpoB genes. The physico-chemical characteristics of GON were analyzed through GC-mass analysis, and Nanotrac- Zeta sizer. The antibacterial activity was studied by minimum inhibitory concentration using the transmission electron microscope. All isolates used were positive for16 SrRNA and rpoB genes. The average Garlic oil nanoemulsion size was 118 nm with major homogeneity. Garlic essential oil nanoemulsion has a negative charge with automatic polarity with 89 us/cm conductivity. Garlic essential oil nanoemulsion was safe to be used and the fatty acids, especially oleic and stearic acids, were its major particulars. Garlic oil nanoemulsion has deleterious effects on T. pyogenes as causes cell lysis, cytoplasmic leakage, and nanoparticle clusters on the bacterial cell. Furthermore, it resulted in alterations to the exterior surfaces, interior characteristics and biological activity of the cells, so it should be studied on a large scale and trial to explore in vivo efficacy of this product should be done.
Keywords
Trueperella pyogenes; 16S rRNA; rpoB gene - Garlic oil nanoemulsion; GC-mass analysis
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