Morphopathological and Biochemical Changes Induced by Cisplatin as Anticancer Drug and The Protective Role of Moringa Leaf Ethanolic Extract and L-Carnitine in Rat | ||
| Journal of Current Veterinary Research | ||
| Article 6, Volume 1, Issue 1, June 2019, Pages 94-102 PDF (2.02 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jcvr.2019.10738.1003 | ||
| Authors | ||
| Hamdy Abd El-aziz Salem1; enas Abd El-aziz Tahoun* 2; anhar ali kamel3 | ||
| 1professor department of pathology,faculty of veterinary medicine, university of sadat city | ||
| 2lecturer, Department of pathology, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, 32897, Egypt. | ||
| 3department of pathology, facultyof veterinary medicine university of sadat city | ||
| Abstract | ||
| Cisplatin is a potent, highly effective, broad spectrum chemotherapeutic agent, its clinical application is limited due to its adverse effects on normal tissues Since the pathogenesis of cisplatin-induced toxicities mainly involve free radical and lipid peroxidation, this study was designed to evaluate the protective efficacy of Moringa leaf ethanolic extract and L-carnitine against cisplatin-nephrotoxicity and hepatotoxicity in rats. 72 male albino rats were divided into 6 groups GI- control received no treatment, GII- cisplatin (7mg/kg for 4 days), GIII- Prophylaxis by Moringa (500mg/kg for 10 days) then cisplatin as in G2, GIV- Prophylaxis by L-Carnitine (200 mg/kg for 10 days) then cisplatin as in G2, GV- treatment by Moringa received cisplatin as group II plus Moringa extract for 14 days. VI- treatment by L-Carnitine received cisplatin as group II plus L-Carnitine for 14 days. The results indicate that cisplatin induced significant increase in serum ALT, AST, Urea, Creatinine and kidney MDA level, also significant decrease in kidney GSH and CAT levels and histopathological alteration in kidney tissues as cloudy swelling, necrobiotic changes of tubular epithelium, congestion of glomerular tuft, also hepatic lesions as congestion of the central and portal vein with necrosis of its lining intimal cells, formation of newly formed bile duct with lymphocytic cell infiltrations in portal area and coagulative necrosis of some hepatocytes and another hepatocytes showed hydropic degeneration. Both moringa leaf ethanolic extract and L-carnitine ameliorated these toxicities as evidenced by restoring normal serum ALT, AST, Urea, Creatinine and histopathological architecture of kidney and liver. | ||
| Keywords | ||
| Key wards: Cisplatin; Moringa; L-carnitine; Oxidative Stress; histopathology | ||
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