GERGIS, A., HAFEZ, M., MOHAMMED, Z., ABDELAZIZ, S., HASSAN, A., GAMAL ABDELAZIZ, S. (2024). PHENOTYPIC AND GENOTYPIC CHARACTERIZATION OF LISTERIA MONOCYTOGENES ISOLATED FROM RAW MILK IN ASSIUT CITY. test, 70(181), 232-243. doi: 10.21608/avmj.2024.253310.1209
AMAL ISHAK GERGIS; MARY REFAT HAFEZ; ZEINAB AHMED MOHAMMED; SAHAR GAMAL ABDELAZIZ; AZHAR MOHAMMED HASSAN; SAHAR GAMAL ABDELAZIZ GAMAL ABDELAZIZ. "PHENOTYPIC AND GENOTYPIC CHARACTERIZATION OF LISTERIA MONOCYTOGENES ISOLATED FROM RAW MILK IN ASSIUT CITY". test, 70, 181, 2024, 232-243. doi: 10.21608/avmj.2024.253310.1209
GERGIS, A., HAFEZ, M., MOHAMMED, Z., ABDELAZIZ, S., HASSAN, A., GAMAL ABDELAZIZ, S. (2024). 'PHENOTYPIC AND GENOTYPIC CHARACTERIZATION OF LISTERIA MONOCYTOGENES ISOLATED FROM RAW MILK IN ASSIUT CITY', test, 70(181), pp. 232-243. doi: 10.21608/avmj.2024.253310.1209
GERGIS, A., HAFEZ, M., MOHAMMED, Z., ABDELAZIZ, S., HASSAN, A., GAMAL ABDELAZIZ, S. PHENOTYPIC AND GENOTYPIC CHARACTERIZATION OF LISTERIA MONOCYTOGENES ISOLATED FROM RAW MILK IN ASSIUT CITY. test, 2024; 70(181): 232-243. doi: 10.21608/avmj.2024.253310.1209

PHENOTYPIC AND GENOTYPIC CHARACTERIZATION OF LISTERIA MONOCYTOGENES ISOLATED FROM RAW MILK IN ASSIUT CITY

Assiut Veterinary Medical Journal
Article 19, Volume 70, Issue 181, April 2024, Pages 232-243    PDF (891.87 K)
Document Type: Research article
DOI: 10.21608/avmj.2024.253310.1209
Authors
AMAL ISHAK GERGIS1; MARY REFAT HAFEZ1; ZEINAB AHMED MOHAMMED2; SAHAR GAMAL ABDELAZIZ* 3; AZHAR MOHAMMED HASSAN* 1; SAHAR GAMAL ABDELAZIZ GAMAL ABDELAZIZ4
1Animal Health Research Institute, (AHRI), Agriculture Research Center (ARC), Assiut Branch
2Animal Health Research Institute, (AHRI), Agriculture Research Center (ARC), Luxor Branch
3Microbiology Department, Animal Health Research Inistitute (AHRI), Agriculture Research Centre (ARC), Qena, Egypt.
4Microbiology Department, Animal Health Research Inistitute (AHRI), Agriculture Research Center (ARC), Qena, Egypt
Abstract
Among a food-associated pathogen, Listeria monocytogenes has become the most important one. It can cause a "high fatality rates 20–30%" in comprasion to another foodborne bacteria which causing dangerous disease called listeriosis. Listeria outbreaks are often linked to dairy products and milk. This article intends to review phenotypic and genotypic characters of L. monocytogenes in Assiut city. How common L. monocytogenes is determined in different sources of raw milk (20 market milk, 40 buffleo milk and 40 cow milk).  By detection of 3 virulence genes and its sequencing (hlyA, inIB and (prfA) can deterimined the pathogenic potential of the isolates. Firstly enrichment samples in fraser broth, then, plating on to ALOA®, Merck. Finally a multiplex-PCR,was used for identification of suspected colonies. The results revealed that 8 (40%), 6 (15%) and 11 (27%) of samples that collected from market, buffalo and cow raw milk were positive respectively. Antibacteerial sensitive test showing in vitro thai Listeria mmonocytogenes had a higher sensitivity to Sulfa methoxazoletrimthoprim (SXT) and Ciprofloxacin, (CIP) followed by Chloramphenicol (C), low sensitive to  Gentamicin (CN), it resisted Erythrommycin (E) and Amoxicillin (AX). The PCR results for isolates have hly A Inl B and prf A genes of L. monoctogenes.
Keywords
raw milk PCR; L. monocytogenes hly A; Inl B and prf A.genes
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