Study of Some Biomarkers Changes in Patients with Lupus Nephritis and Their Correlation with Disease Activity and Progression | ||
| Bulletin of Egyptian Society for Physiological Sciences | ||
| Article 2, Volume 33, Issue 1, June 2013, Pages 17-34 PDF (364.65 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/besps.2013.34884 | ||
| Authors | ||
| Walaa Keshk* 1; Nema Soliman1; Noha Esheba2 | ||
| 1Department of Medical Biochemistry, Faculty of Medicine, Tanta University | ||
| 2Department of Internal Medicine, Faculty of Medicine, Tanta University | ||
| Abstract | ||
| Background: Systemic lupus erythematosus (SLE) is a chronic multisystem autoimmune disease characterized by complex clinical manifestations and chronic inflammatory processes with failure of immunoregulatory mechanisms. Kidney is one of the most commonly affected organs. Considering the morbidity associated with SLE and particularly with lupus nephritis (LN), it is important to identify novel biomarkers of disease activity which could aid in the detection and assessment of flares and degree of activity. At present, activity of SLE is assessed based on clinical symptoms and biochemical parameters such as autoantibody (e.g antinuclear antibody (ANA)) and serum complement. However, these biomarkers are not specific for evaluating renal activity. Renal biopsy is the gold standard for assessment of kidney damage and disease activity, but its usage is restricted as it is an invasive procedure. Aim of the work: In the present study, plasma level of advanced oxidation protein products (AOPPs) and peripheral blood mononuclear cells nuclear factor-κB (PBMCs NF-κB) level as well as serum levels of fetuin-A, 25-hydroxyvitamin D (25OHD), calcium (Ca), inorganic phosphate were studied as novel biomarkers of LN activity and progression. Methods: The study included 30 SLE female patients, 15 without nephritis (group II) and 15 with nephritis (group III), in addition to 15 agematched healthy controls (group I). Overnight fasting blood was collected from all subjects for measurement of plasma AOPPs level, PBMCs NF-κB level and serum fetuin-A level, 25OHD level, Ca and inorganic phosphate levels as well as calculation of calcification risk index (CRI). Results: Plasma AOPPs, PBMCs NF-κB, serum inorganic phosphate levels and CRI were significantly higher in SLE patients (group II) than age-matched healthy controls (group I) (p<0.05) with the highest level in patients with LN (group III) meanwhile, serum fetuin-A and 25OHD levels were significantly lower in SLE patients than age-matched healthy controls (p<0.05) with the lowest level in LN patient group. In addition plasma AOPPs, PBMCs NF-κB levels and CRI showed significantly positive correlation meanwhile, fetuin-A and 25OHD levels showed significantly negative correlation with serum creatinine, 24 hours urinary albumin, erythrocyte sedimentation rate (ESR), C reactive protein (CRP), ANA, anti double stranded DNA (Anti- dsDNA) antibodies levels and SLE disease activity index (SLEDAI). Conclusions: In SLE patients, high PBMCs NF-κB and plasma AOPPs levels as well as CRI while low levels of fetuin-A and 25OHD were related to disease activity and progression. | ||
| Keywords | ||
| Systemic lupus erythematosus (SLE); lupus nephritis (LN); Advanced oxidation protein products (AOPPs); Peripheral blood mononuclear cells nuclear factor-κB (PBMCs NF-κB); Fetuin-A; 25-hydroxyvitamin D (25OHD); Calcification risk index (CRI) | ||
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