Study of Galectin 3 and Matrix Metalloproteinase -9 as Prognostic Markers in Colon Cancer | ||
| Bulletin of Egyptian Society for Physiological Sciences | ||
| Article 3, Volume 33, Issue 1, June 2013, Pages 35-52 PDF (472.7 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/besps.2013.34888 | ||
| Authors | ||
| Mohamed Badawy* 1; Iman Abdelgawad1; Asmaa Abdelgawad2 | ||
| 1Department of Clinical pathology, Faculty of Science - Cairo University, Egypt | ||
| 2Department of NCI, Biochemistry, Faculty of Science - Cairo University, Egypt | ||
| Abstract | ||
| Objectives: The present study was carried out to evaluate the diagnostic role of galectin 3 and matrix metalloproteinase 9 in 50 consecutive newly diagnosed colon cancer patients, who presented to the Outpatient Clinic of the NCI over the period 2011-2012 and to compare their serum levels before treatment with those of 15 benign colon lesions patients, 15 normal controls subjects, and in 16 patients of the cancer colon group after treatment. Also, to compare these studied markers with CEA, CA 19.9 and some prognostic factors of cancer colon. Methods: Serum concentration of CEA and CA19.9 were evaluated using Axsym and MMP9 and Galectin 3 antigen were assessed using ELISA technique. Results: Serum levels of MMP9, CEA ,and CA 19.9 showed highest results in the malignant group before treatment, followed by the patient group with benign lesion, then the malignant group after treatment and lastly comes the control group. While galectin 3 showed the highest results in the malignant group, followed by the benign then the control and lastly comes the after treatment group. Diagnostic performance of all the studied markers at the chosen cutoffs, CEA (21 ng/ml), CA19.9 (58 U /ml), MMP9 (90.9 ng/ml) and galectin 3 (4.8 ng/ml),galectin 3 showed the highest sensitivity, followed by CEA, and then CA19.9 and lastly MMP9 (96.9%, 92.3%, 81.5% and 75.4% respectively). As regards specificity % CEA, CA19.9 and MMP9 showed 100% specificity each, while galectin 3 showed relatively lower specificity % (90.3%). Galectin 3 and CEA showed comparable high diagnostic accuracy (94.8%) followed by CA19.9 (87.5%) then MMP9 (83.3%). On comparing diagnostic performance for the studied tumor markers in double combinations, the best sensitivity (98.5 %) was obtained when combining galectin 3 & CA19.9 at cut offs (4.8 ng/ml - 58 U /ml) respectively and galectin 3 & MMP9 at cutoffs (4.8 ng/ml – 90.9ng/m) respectively. As regards specificity, the double combination between CEA & CA19.9 at cut offs (21 ng/ml - 58 U /ml)) respectively and MMP9 & CA19.9 at cut offs (90.9 ng/ml - 58 U /ml) respectively showed the highest specificity of 100% each, on studying the correlation between all the studied tumor markers, there were significant positive correlations between CEA & CA19.9 , CEA & Galectin 3, CEA & MMP9, CA19.9 & Galectin 3, CA19.9 & MMP9 and MMP9 & Galectin 3 (p=0.0001) each. | ||
| Keywords | ||
| galectin 3; MMP9; Colon cancer; tumor marker | ||
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