Antibacterial Effect of Different Vitamin C Formulations on Multi-drug Resistant Salmonella Serovars Recovered from Broiler Chickens | ||
| Journal of Veterinary Medical Research | ||
| Article 1, Volume 31, Issue 1, June 2024, Pages 1-8 PDF (1.39 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jvmr.2024.271111.1098 | ||
| Authors | ||
| Ismail Abd El-hafeez Raheel1; Marwa Mostafa Mahmoud* 2; Ahmed Hussein Abed1; Ahmed Elbestawy3 | ||
| 1Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Beni-Suef University, Beni Suef 62511, Egypt. | ||
| 2Postgraduate at Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Beni-Suef University, Beni Suef 62511, Egypt. | ||
| 3Department of Bird and Rabbit Diseases, Menoufia University, Shebeen Elkom 32511, Menoufia, Egypt. | ||
| Abstract | ||
| There is a great exertion to look for alternative therapeutic methods that assist in controlling the antibacterial resistance of Salmonellae. This study aimed to evaluate the antibacterial effect of vitamin C (ascorbic acid) in different formulations at different concentrations. Serotyping of the isolates revealed that 10 belonged to S. Enteritidis 1,9,12:g,m, 7 S. Virchow 6,7,14:r:1,2, and 3 S. Montevideo 6,7,14:g,m[p],s :[1,2,7]. The isolates were highly resistant (100%) to amoxicillinclavulanic acid, streptomycin, erythromycin, clindamycin, and doxycycline, and 90% of the isolates were susceptible to amikacin. PCR revealed that 10 S. Enteritidis isolates harbored 3 virulence-associated genes invA, sefA, and fimH, while 7 isolates harbored stn gene. Also, 10 S. Enteritidis isolates harbored sul1 and blaTEM genes. Biofilm formation was 100%, 50%, and 42.8% in S. Virchow, S. Enteritidis, and S. Montevideo, respectively. Vitamin C (formulation 1) completely inhibited the growth of all isolates at concentrations of 0.3%, 0.5%, and 1%. Formulation 2 inhibited the growth of the tested isolates at a concentration of 0.3%. While a concentration of 0.1% inhibited only the growth of S. Virchow. Formulation 3 inhibited the growth of S. Enteritidis and S. Virchow at concentrations of 0.3 and 0.5%. Formulation 4 inhibited the growth of S. Enteritidis, S. Virchow, and S. Montevideo at a concentration of 1%. Formulation 5 inhibited the growth of S. Enteritidis and S. Virchow at concentrations 0.1%, 0.5%, and 1%. Formulations 4 and 1 inhibited effectively the biofilm-forming ability of S. Enteritidis (80% and 100%) and S. Virchow (60% and 66.6%). | ||
| Keywords | ||
| Antimicrobials; Organic Acids; Salmonella; Vitamin C | ||
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