Assessing Shelf-life Ripening of Newly Imported Greenhouse Tomato Cultivars in Saudi Arabia: A qPCR Approach | ||
| Egyptian Journal of Botany | ||
| Article 26, Volume 64, Issue 2, May 2024, Pages 765-772 PDF (860.59 K) | ||
| Document Type: Regular issue (Original Article) | ||
| DOI: 10.21608/ejbo.2024.264251.2672 | ||
| Author | ||
| Bassam Alowaiesh* | ||
| Biology Department, College of Science, Jouf University, Sakaka, Aljouf 72341, Saudi Arabia | ||
| Abstract | ||
| fruit firmness is highly associated with shelf life. In tomato fruits (Solanum lycopersicum L. cv. ‘Myrock’), ethylene is responsible for the loss of firmness associated with climacteric fruit. Ethylene biosynthetic pathway is governed by two families of genes coding for 1-aminocyclopropane-1-carboxylate oxidase (ACO) and 1-aminocyclopropane-1-carboxylate synthase (ACS). In the current study, the gene expression of the ACO gene (LeACO1) and the ACS gene (LeACSA) with a housekeeping gene (LeGAPDH) were investigated for their influence on shelf life and ethylene production in three different tomato varieties (Roma, Sakura F1, and GS12), and quantified using the quantitative polymerase chain reaction method (qPCR). The genetic diversity in the molecular regulation for the perception of ethylene biosynthesis during and after fruit ripening was investigated on the three tomato cultivars in three different maturity stages, mature green (MG), breaker (BR), and full red (FR). The obtained results suggested that the LeACSA gene ethylene production is responsible for revealing the stage of FR tomato in all tested tomato species by the significant accumulation of ACS mRNA in the fruit. The expression of the LeACO1 gene in tomato variety Roma was decreased in full red-ripe (FR) tomato fruits than in the BR and MG stages, respectively. However, in Sakura F1, the expression of LeACSA and LeACO1 were decreased in the full red stage, which showed the lowest ethylene production and the most extended shelf life, which could be used in tomato breeding programs with local cultivars to provide a core cultivar with the most extended shelf-life duration. | ||
| Keywords | ||
| 1-aminocyclopropane-1-carboxylic acid oxidase (ACO); 1-aminocyclopropane-1-carboxylic acid synthase (ACS); Ethylene Biosynthesis; qPCR; Shelf-Life; Tomato | ||
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