Hamed, M., Abo Zakaib Ali, F., Mahmoud, A., Kh. Abd-Elghaffar, S. (2024). Immunohistochemical Expression of MAC387, Arginase 1 and CD163 in Tuberculous Granuloma of Imported Beef Cattle.. test, 2(1), 1-9. doi: 10.21608/ijcvr.2024.353316
Mohamed. G. Hamed; Fatma Abo Zakaib Ali; A.Z. Mahmoud; Sary Kh. Abd-Elghaffar. "Immunohistochemical Expression of MAC387, Arginase 1 and CD163 in Tuberculous Granuloma of Imported Beef Cattle.". test, 2, 1, 2024, 1-9. doi: 10.21608/ijcvr.2024.353316
Hamed, M., Abo Zakaib Ali, F., Mahmoud, A., Kh. Abd-Elghaffar, S. (2024). 'Immunohistochemical Expression of MAC387, Arginase 1 and CD163 in Tuberculous Granuloma of Imported Beef Cattle.', test, 2(1), pp. 1-9. doi: 10.21608/ijcvr.2024.353316
Hamed, M., Abo Zakaib Ali, F., Mahmoud, A., Kh. Abd-Elghaffar, S. Immunohistochemical Expression of MAC387, Arginase 1 and CD163 in Tuberculous Granuloma of Imported Beef Cattle.. test, 2024; 2(1): 1-9. doi: 10.21608/ijcvr.2024.353316

Immunohistochemical Expression of MAC387, Arginase 1 and CD163 in Tuberculous Granuloma of Imported Beef Cattle.

International Journal of Comprehensive Veterinary Research
Volume 2, Issue 1, June 2024, Pages 1-9    PDF (1.3 M)
Document Type: Original Article
DOI: 10.21608/ijcvr.2024.353316
Authors
Mohamed. G. Hamed* 1; Fatma Abo Zakaib Ali1; A.Z. Mahmoud2; Sary Kh. Abd-Elghaffar3
1Department of Pathology and Clinical Pathology, Faculty of Veterinary Medicine, Sohag University
2Department of Pathology and Clinical Pathology, Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt,
3Department of Pathology and Clinical Pathology, Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt. Department of Pathology and Clinical Pathology, School of Veterinary Medicine, Badr University, Assiut, Egypt.
Abstract
Macrophages are the first immune cells to interact with inhaled bacilli, and they have the ability to suppress bacterial growth through phagocytosis. Two distinct phenotypes can be found within granuloma. First, mycobacteria are eliminated more quickly and are killed by proinflammatory (M1). Second, anti-inflammatory macrophages (M2), are intended to preserve tissue integrity and support tissue repair. Therefore, the current study used histopathological examination to classify tuberculous granuloma into four stages (stage I to stage IV) and immunohistochemistry (IHC) to investigate the expression of MAC387, CD163, and Arginase 1 in naturally infected Beef cattle with tuberculosis at different Stages of TB granulomas. Immunohistochemical analysis revealed that the immunolabeling of MAC387+ macrophages significantly reduced as the stage of granuloma increased from stage I to stage IV (P <0.008). However, the immunolabeling of Arg1+ and CD163+ macrophages significantly increased as the stage of granuloma increased from stage I to stage IV (P<0.01, and P<0.001, respectively).
Keywords
Arginase 1; Beef cattle; CD163; Granuloma; TB
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