In vitro MICROTUBERIZATION OF SOME POTATO (Solanum tuberosum L.) CULTIVARS AS RESPONSE TO MEDIA CONSTITUENTS AND PHOTOPERIOD | ||
| Journal of Productivity and Development | ||
| Article 2, Volume 29, Issue 2, April 2024, Pages 81-98 PDF (774.57 K) | ||
| Document Type: Original Article | ||
| Authors | ||
| Amina Wafa1; Wafaa Fekry* 2; Aly Hassan2; Ismail Khatab3 | ||
| 1Plant Prod. Dept., Fac. Technol. and Dev., Zagazig Univ., Egypt | ||
| 2Plant Prod. Dept., Fac. Technol. and Dev., Zagazig Univ., Egypt. | ||
| 3Genetics Dept., Fac. Agric., Kafr El- Sheikh Univ., Egypt. | ||
| Abstract | ||
| This work was carried out in cooperation with the plant production Dept., Fac. Technol. and Develop., Zagazig Univ., andGenetic Engineering and Tissue CultureLab., Genetics Dept., Fac. Agric., Kafr El- Sheikh Univ., during the period from 2020 to 2022 to study the performance of potato (Solanum tuberosum L.) genotypes, i.e. Diamant and Spunta for producing in vitro microtubers. The effect of media constituents, i.e. various combinations between kinetin and sucrose concentrations, as well as incubation photoperiod conditions were observed in two separate experiments. These experiments were arranged with Randomized complete design (RCD) with three replicates. As for the first experiment, there were fourteen treatments used, two potato cultivars with seven media combinations, i.e. 3, 6 and 9% sucrose without Kin or 6 and 9% sucrose either with 2 or 3 mgl-1 Kin. Meanwhile, in the other experiment, double nodes segment explants for both cultivars were cultured on MS medium (0.75% strength) contained 3 mgl-1 Kin + 6% sucrose and incubated under three photoperiods: 1) 16h/8h (light/dark), 2) 8h/16h (light/dark) and 3) full darkness (six treatments) two experiments were conducted in RCD with three replicates. The obtained results revealed that Diamant cultivar was more response to treatments than Spunta cv. MS medium supplemented with 3 mgl-1 Kin + 6 or 9% sucrose reduced the number of days to microtuber induction, increased tuberization percentage, number of microtubers after 30 and 75 days (at harvest) as well as average of microtubers fresh weight. Diamant cv. achieve the highest results for these parameters. Moreover, incubation cultures under photoperiod condition 8h/16h (light/ dark) or darkness provides best results as compared with light condition especially with Diamant cultivar. Conclusively, it can be concluded that increasing Kin up to 3 mgl-1 + 6 or 9% sucrose with MS medium (0.75% strength) and incubated explants in short day or darkness particularly with diamante cv. enhanced and improved the production and development of in vitro microtubers. | ||
| Keywords | ||
| Potato; tuberization; sucrose; kinetin; photoperiod; microtubers | ||
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