Biosynthesis of silver nanoparticles using cow dung extract and evaluation of their antibacterial potentials | ||
| Microbes and Infectious Diseases | ||
| Articles in Press, Accepted Manuscript, Available Online from 19 May 2024 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mid.2024.281553.1879 | ||
| Authors | ||
| Manoja Das* ; Dipti Kanta Acharya; Malati Hembrom; Ipsita Nayak | ||
| Department of Biotechnology, GIET University, Gunupur-765022, Odisha, India | ||
| Abstract | ||
| Cow dung, also called cow pies or cow manure, is the excrement left behind by bovine animals, such as buffalo, yak, and cows. The work aims to green synthesize the silver nanoparticles accomplished by aqueous extract from cow dung. To convert AgNO3 into silver nanoforms, After mixing dung extract with 100 mM silver nitrate (AgNO3), the mixture was incubated for 24 hours at 27 ± 2o C. The first indication that the green synthesis of nanoforms had occurred was the combination solution's hue changing from slightly yellow to brown after incubation. The biogenesis of silver nanoforms was further validated by transmission electron microscopy (TEM), UV-VIS spectrophotometer analysis, and X-ray diffraction (XRD). The functionality groups of nanoforms are ascertained using Fourier Transform infrared (FTIR) spectroscopy. The nanoforms had the absorption maxima (ʎ max) at 442 nm. The TEM photography indicated that the biosynthesised nanoforms were nearly rounded structure-functional groups. It revealed that the nanoparticles' average size was 39 nm. The ideal mixture of raw material (AgNO3) and the extract was found to be 9:1. The alkaline solution (pH 8.5) favours the conversation process. Moreover, the antibacterial properties of the generated nanoparticles against three pathogens were examined using the agar well diffusion experiment, namely Escherichia coli, Bacillus subtilis, and Staphylococcus aureus. The result indicated that the synthesised nanoforms had the efficacy to inhibit all the studied microorganisms, which is attributed to the therapeutic use of the nanoforms. | ||
| Keywords | ||
| Green synthesis; silver nano forms; cow dung; antimicrobial properties | ||
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