salman, N., Anwer, Z., Mostafa, H., khairy, G. (2024). The Effect of Caffeine on the interaction between Bovine Serum Albumin and Losartan potassium by Spectroscopic Methods. test, 9(1), -. doi: 10.21608/fsrt.2024.282853.1125
nada ali salman; Zeinab mohamed Anwer; Hend Mohamed Mostafa; Gasser mohamed khairy. "The Effect of Caffeine on the interaction between Bovine Serum Albumin and Losartan potassium by Spectroscopic Methods". test, 9, 1, 2024, -. doi: 10.21608/fsrt.2024.282853.1125
salman, N., Anwer, Z., Mostafa, H., khairy, G. (2024). 'The Effect of Caffeine on the interaction between Bovine Serum Albumin and Losartan potassium by Spectroscopic Methods', test, 9(1), pp. -. doi: 10.21608/fsrt.2024.282853.1125
salman, N., Anwer, Z., Mostafa, H., khairy, G. The Effect of Caffeine on the interaction between Bovine Serum Albumin and Losartan potassium by Spectroscopic Methods. test, 2024; 9(1): -. doi: 10.21608/fsrt.2024.282853.1125

The Effect of Caffeine on the interaction between Bovine Serum Albumin and Losartan potassium by Spectroscopic Methods

Frontiers in Scientific Research and Technology
Volume 9, Issue 1, September 2024    PDF (654.5 K)
Document Type: Original Article
DOI: 10.21608/fsrt.2024.282853.1125
Authors
nada ali salman* 1; Zeinab mohamed Anwer2; Hend Mohamed Mostafa3; Gasser mohamed khairy4
1Basic Science Department ,Faculty of Dentistry ,Sinai University ,El-Arish, North Sinai
2Professor of Analytical Chemistry Faculty of Science Suez Canal University
3chemistry department ,Faculty of Science, El-Arish University, AL-Arish,Egypt
4Associate Professor of Analytical Chemistry Chemistry Department, Faculty of science Suez University, Suez, Egypt
Abstract
Losartan potassium (LP) is an antihypertensive drug, and its binding to plasma proteins determines its pharmacokinetic properties. The presence of an exogenous substance like Caffeine (CAF) may affect the binding of drugs to proteins. Accordingly, we aim to characterize LP and bovine serum albumin (BSA) interactions in CAF presence employing spectroscopic techniques (UV-vis) absorption and fluorescence measurements) under physiological circumstances. Our findings indicated that the quenching effect of LP on BSA was decreased by adding CAF by calculating the Stern-Volmer constant. Hence, the Modified Stern-Volmer equation was utilized for calculating binding constants for the BSA-LP and BSA-CAF-LP systems, and the values were 4.47×103and 0.23×103, respectively. Thermodynamic parameters, the Gibbs free energy (∆G), enthalpy (∆H), and entropy (∆S) calculated for each system show spontaneous interaction for both systems and suggest that the binding interaction between BSA and LP is exothermic and entropy is unfavorable, while for BSA-CAF-LP is endothermic and entropy is favorable.
Keywords
Absorption; Fluorescence; Bovine serum albumin; competitive binding
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