HSP70 gene Polymorphism in Pre-eclamptic Women and its Correlation with Serum Levels of HSP70 | ||
| Bulletin of Egyptian Society for Physiological Sciences | ||
| Article 14, Volume 32, Issue 1, June 2012, Pages 207-218 PDF (200.47 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/besps.2012.35598 | ||
| Authors | ||
| Ayman Darwish* 1; Burhan Imamwerdi2; Mahmoud Melis3; Fathia AlJama4 | ||
| 1Department of Medical Biochemistry, Faculty of Medicine, Alexandria University, Egypt | ||
| 2Department of Medical Lab. Technology, College of Applied Medical Sciences, King Faisal University, KSA | ||
| 3Department of Obstetrics & Gynaecology, Faculty of Medicine, Alexandria University, Egypt | ||
| 4Department of Obstetrics & Gynaecology , College of Medicine, King Faisal University, KSA | ||
| Abstract | ||
| Objective: The present study was designed to evaluate the possible association of three different HSP70; HSPA1A, HSPA1B and HSPA1L; gene polymorphisms with pre-eclampsia and the possible correlation with serum levels of HSP70. Method: The study included 46 women with pre-eclampsia as group I (GI) and twenty two normotensive pregnant controls representing group II (GII). All candidates of the study were subjected to the following: Measurement of human HSP70 concentrations in serum, molecular analysis for PCR amplification of the HSPA1A G (190)C regions, HSPA1B A(1267)G regions, HSPA1L T(2437)C regions. Results: Serum HSP70 was found to be significantly increased in pre-eclamptic patients when compared to normal pregnancy. HSPA1A (190) GC and HSPA1B (1267) GG genotypes occurred more frequently in pre-eclamptic patients compared to healthy controls (p < 0.03). Significant difference was found in the distribution of HSPA1B (1267) AG genotype between the pre-eclamptic and control group (p < 0.004). Distribution of HSPA1L T(2437)C gene was found similar in the Pre-eclamptic and control group. Cases with HSPA1A G(190)C had a mean serum level of HSP70 that is 1.4 ng/ml more than those without HSPA1A G(190)C polymorphism. A significant negative association was noted between maternal age and serum Hsp70 concentration in both preeclampsia and healthy pregnant women. Conclusion: Elevated serum HSP70 level in pre-eclamptic patients seems to reflect the burden of oxidative stress taking place im pre-eclampsia. HSP70 polymorphism may affect the serum level as proved by increase in the mean serum level of HSP70 by 1.4 ng/ml in those with HSPA1A G(190)C polymorphism . | ||
| Keywords | ||
| Pre-eclampsia; Heat shock proteins; Heat Shock Protein 70; Polymorphisms | ||
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