Therapeutic Potential of Hyaluronic acid hydrogel /Bone marrow stem cell-conditioned medium on Articulating Surfaces Associated with Induced Arthritis in Rabbit’s TMJ | ||
| Al-Azhar Assiut Dental Journal | ||
| Volume 7, Issue 1, April 2024, Pages 49-55 PDF (1.16 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/aadj.2024.273451.1165 | ||
| Authors | ||
| mai mahmoud assi* 1; samah khaled1; Mohammed Grawish2; mohammed elsayed helal1 | ||
| 1oral biology. Faculty of dentistry .Mansoura university. Mansoura | ||
| 2Faculty of Oral and Dental Medicine, Delta University for Science and Technology, Gamasa, Mansoura, Egypt. | ||
| Abstract | ||
| Aim: To assess hyaluronic acid hydrogel /bone marrow stem cell-conditioned medium (MSC-CM with HA hydrogel) on articulating surfaces associated with induced arthritis in rabbit’s TMJ. Subjects and methods: , fifty rabbits were employed. A technique to induce arthritis was performed on forty rabbits using a double IA injection of CFA. There were made five groups of rabbits. 10 rabbits in Group I (the normal group) and 10 in Group II (the arthritic group) did not receive any treatment, respectively. Ten animals each got a bilateral IA injection of 0.2 ml of Group III hyaluronic acid hydrogel.10 rabbits each got a bilateral IA injection of 0.2 ml of Group IV and bone marrow stem cell condition media.10 rabbits were given bilateral IA injections of 0.2ml of a combination therapy (hyaluronic acid hydrogel and bone marrow stem cell condition medium (Group V). After the fourth week of injection-based therapy, all rabbits will be anesthetized then euthanized. Dissection, decalcification, and preparation Aim: To assess hyaluronic acid hydrogel /bone marrow stem cell-conditioned medium (MSC-CM with HA hydrogel) on articulating surfaces associated with induced arthritis in rabbit’s TMJ. Subjects and methods: , fifty rabbits were employed. A technique to induce arthritis was performed on forty rabbits using a double IA injection of CFA. There were made five groups of rabbits. 10 rabbits in Group I (the normal group) and 10 in Group II (the arthritic group) did not receive any treatment, respectively. Ten animals each got a bilateral IA injection of 0.2 ml of Group III hyaluronic acid hydrogel.10 rabbits each got a bilateral IA injection of 0.2 ml of Group IV and bone marrow stem cell condition media.10 rabbits were given bilateral IA injections of 0.2ml of a combination therapy (hyaluronic acid hydrogel and bone marrow stem cell condition medium (Group V). After the fourth week of injection-based therapy, all rabbits will be anesthetized then euthanized. Dissection, decalcification, and preparation of the mandible for Masson’s Tri-chrome staining. Results: Those different treatment modalities responded to osteoarthritis in different ways, with MSC-CM with HA hydrogel being regarded as the most effective treatment of induced temporomandibular joint osteoarthritis among the tested treatment modalities. Conclusion: It was possible to conclude, within the limitations of the current study, MSC-CM with HA hydrogel being regarded as the most effective treatment of induced temporomandibular joint osteoarthritis | ||
| Keywords | ||
| TMJ; osteoarthritis; hyaluronic acid hydrogel; bone marrow stem cell; articulating surfaces | ||
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