Indirect Micropropagation of Gerbera jamesonii Bolus cv. Winter Queen through Callus Redifferentiation | ||
| Journal of Plant Production | ||
| Article 1, Volume 9, Issue 4, April 2018, Pages 307-314 PDF (1.09 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jpp.2018.35686 | ||
| Author | ||
| M. Kasem* | ||
| Department of Vegetable and Floriculture, Faculty of Agriculture, Mansoura Univ., Egypt | ||
| Abstract | ||
| This research was achieved in the Laboratory of Plant Tissue Culture, Vegetable and Floriculture Department, Mansoura University, Egypt during 2015/2016 on Gerbera jamesonii Bolus cv. Winter Queen for studying the indirect micropropagation of this important pot and cut flower plant in Egypt. For callus induction, unopened flower buds(10 days old) were cultured after preparation on MS medium fortified with either 2,4-D or NAA (0.5, 1.0, 2.0, 4.0 and 6.0 mg/l) each alone. The calli were transferred on media supplemented with either BAP or meta-topolin (0.0, 0.3, 0.6, 1.2 and 2.4 mg/l) each alone with 0.5 mg/l NAA as a fixed concentration for redifferentiation. Moreover, shoots were transferred to media supplemented with NAA (0.0, 0.5, 1.0, 1.5 and 2.0 mg/l) with 0.3 mg/l meta-topolin for the in vitro rooting. Results showed that 6.0 mg/l 2,4-D produced the heaviest callus fresh weight (7.22 g), and decreased the callus induction time (17.50 days), but the callus texture was compact and creamish to brown in color. In addition, the lower concentrations from either 2,4-D (1.0 and 2.0 mg/l) or NAA (0.5 to 1.0 mg/l) produced a nodular greenish calli with greenish color. Also, supplying the redifferentiation media with 1.2 and 2.4 mg/l meta-topolin or 2.4 mg/l BAP, significantly reduced the redifferentiation time, increased the multiplication percentage and micro-shoots number. Moreover, ½ strength MS medium fortified with 2.0 mg/l NAA+0.3 mg/l meta-topolin gave the maximum roots number, rooting% and reduced the roots initiation time. Finally, well rooted plantlets were transferred to a mixture of peatmoss+perlite+clay (2:1:1 v/v) produced a survival percentage of 92.90% with a plantlets height of 4.61cm and 4.50 leaves / plantlet on average. | ||
| Keywords | ||
| callus dedifferentiation; callus redifferentiation; callus induction; multiplication; in vitro rooting | ||
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