Pilot Study on Molecular Quantitation and Sequencing of Endometrial Cytokines Gene Expression and their Effect on the Outcome of In-Vitro Fertilization (IVF) Cycle | ||
| Bulletin of Egyptian Society for Physiological Sciences | ||
| Article 2, Volume 32, Issue 2, December 2012, Pages 17-30 PDF (551.25 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/besps.2012.35789 | ||
| Authors | ||
| Dina Sabry* 1; Samar Marzouk1; Amira Hassouna2; Olfat Nouh3; Hoda Abdel Aal3 | ||
| 1Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Egypt | ||
| 2Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Egypt | ||
| 3Department of Obstetrics and Gynaecology, Faculty of Medicine, Cairo University, Egypt | ||
| Abstract | ||
| Background: Human trophoblast invasion and differentiation are essential for successful pregnancy outcome. The molecular mechanisms; however, are poorly understood. Interleukin (IL)-11, a cytokine that regulates endometrial epithelial cell adhesion, trophoblast motility and invasion during implantation, may be involved in some of these processes. Leukemia inhibitory factor (LIF) is one of the key cytokines in the embryo implantation regulation. The present study aimed to assess the levels of LIF, IL-11 and IL-11 α receptor gene expression in the endometrium of women undergoing IVF, and correlate their levels with the IVF pregnancy outcome. Also, the study aimed to detect any sequence mutation in these three genes among IVF pregnant and non-pregnant women versus control menstrual blood of fertile women. Methods: Endometrial tissue biopsies were taken from 15 women undergoing IVF on the day of oocyte retrieval. The quantitative expression of IL-11, IL-11Rα and LIF genes were assessed by real time PCR and PCR products were sequenced by DNA sequencer to detect any DNA sequence changes. Menstrual blood from 10 fertile women was used as control to compare the DNA sequence versus DNA sequence of the studied genes in endometrial biopsies. Luteinizing hormone (LH), follicular stimulating hormone (FSH) and estradiol (E2) were assessed for enrolled patients by ELISA. Endometrial thickness was also assessed by pelvic ultrasonography. Results: No significant difference was detected between quantitative expression of the three studied genes and pregnancy IVF outcome. Although DNA sequence changes were found in IL-11 and LIF genes of women with negative pregnancy IVF outcome compared to women with positive pregnancy IVF outcome. No DNA sequence changes were detected for IL-11Rα. Other studied parameters (age, LH, FSH, E2 and endometrial thickness) showed no significant differences or correlation with quantitative expression of the three studied involved genes. Conclusions: Data suggested that no significant differences between quantitative expression of IL-11, IL- 11Rα and LIF genes and the IVF pregnancy outcome. The present study may reveal that changes in IL-11 and LIF genes sequence may contribute in pregnancy IVF outcome. | ||
| Keywords | ||
| Gene expression; interleukin-11 (IL-11); interleukin-11 receptor α (IL- 11Rα); leukemia inhibitory factor (LIF); IVF; DNA sequence | ||
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