Comparison between Circulating Endothelial Cells and Platelet Microparticles Levels in Egyptians Rheumatic Mitral Diseases with and without Atrial Fibrillation | ||
| Bulletin of Egyptian Society for Physiological Sciences | ||
| Article 5, Volume 32, Issue 2, December 2012, Pages 57-69 PDF (356.99 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/besps.2012.35813 | ||
| Authors | ||
| Naglaa Idriss* 1; Hosny Hassen1; Douaa Sayed2; Yehia Keshek3; Marwa Gaber4 | ||
| 1Medical Biochemistry Department, Assiut University, Faculty of Medicine, | ||
| 2Clinical Pathology, South Egypt Cancer Institute | ||
| 3Cardiology Department, Assiut University, Faculty of Medicine | ||
| 4Medical Biochemistry Department, Assiut University, Faculty of Medicine | ||
| Abstract | ||
| Background: Atrial fibrillation (AF) is a continued cardiac arrhythmia, which is linked to a high risk thromboembolism. The association of AF and valvular heart disease results in a substantial stroke and thromboembolic risk. AF is interrelated with a hypercoagulable state and abnormalities of plasma indices of endothelial damage/dysfunction such as circulating endothelial cells (CECs) and platelet microparticles (PMPs), the levels of which are elevated in patients with coronary artery disease (CAD), diabetes mellitus `and stroke, all of which are conditions that lead to (AF). Thus, we hypothesized that the levels of CECs and PMPs would be raised in rheumatic atrial fibrillation diseases. Methods: 60 patients with rheumatic valvular diseases [(30 patients (35±12 years; 10 male and 20 females)] with rheumatic mitral valve disease [(Mitral stenosis MS and Mitral regurgitation MR)] and with normal sinus (RNAF) compared to [(30 patients (31±11 years; 11 male and 19 females)] with rheumatic valvular diseases with AF (RAF) and 20 healthy controls HC (31±10 years; 17 male and 3 females). CECs were calculated using flow cytometry after staining with mouse anti-human CD45 antibody (pan-leukocyte marker), mouse anti-human CD 146 antibody (endothelial cell marker). PMPs were distinguished from non-platelet events by their fluorescence from Fluorescein 6- isothiocyanate (FITC) conjugated anti-CD41a bound to the particle surfaces. Results: RAF patients had significantly elevated numbers of CECs and PMPs compared to healthy controls HC (P<0.0001). CECs levels were significantly higher in RAF diseases compared to patients with RNAF (P<0.05). RAF patients had significantly higher levels of PMPs (p <0.001) compared to HC. Conclusion: Severe endothelial damage, as assessed by increased numbers of CECs, not to be a prominent trait of rheumatic valvular lesions in sinus rhythm patients, however levels are definitely high in rheumatic valvular diseases with AF. There is evidence of platelet activation in rheumatic AF patients, owing to original cardiovascular diseases rather than the arrhythmia as such. | ||
| Keywords | ||
| Platelet microparticles; Circulating endothelial cells; Atrial Fibrillation; endothelial dysfunction | ||
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