Ibrahim, A., Hegab, O., Soliman, N. (2024). Effect of Applying Clove and Cinnamon Essential Oils to Milk Rice Pudding in Controlling Bacillus cereus and Bacillus subtilis Growth with Respect to the Sensory Traits. test, 9(3), 41-49.
Aml S. Ibrahim; Ola W. Hegab; Neveen S.M. Soliman. "Effect of Applying Clove and Cinnamon Essential Oils to Milk Rice Pudding in Controlling Bacillus cereus and Bacillus subtilis Growth with Respect to the Sensory Traits". test, 9, 3, 2024, 41-49.
Ibrahim, A., Hegab, O., Soliman, N. (2024). 'Effect of Applying Clove and Cinnamon Essential Oils to Milk Rice Pudding in Controlling Bacillus cereus and Bacillus subtilis Growth with Respect to the Sensory Traits', test, 9(3), pp. 41-49.
Ibrahim, A., Hegab, O., Soliman, N. Effect of Applying Clove and Cinnamon Essential Oils to Milk Rice Pudding in Controlling Bacillus cereus and Bacillus subtilis Growth with Respect to the Sensory Traits. test, 2024; 9(3): 41-49.

Effect of Applying Clove and Cinnamon Essential Oils to Milk Rice Pudding in Controlling Bacillus cereus and Bacillus subtilis Growth with Respect to the Sensory Traits

Journal of Applied Veterinary Sciences
Article 6, Volume 9, Issue 3, July 2024, Pages 41-49    PDF (535.21 K)
Document Type: Original Article
Authors
Aml S. Ibrahim* ; Ola W. Hegab; Neveen S.M. Soliman
Department of Food Hygiene and Control, Faculty of Veterinary Medicine, Cairo University, 12211, Giza, Egypt
Abstract
Milk rice pudding (MRP) is a commercial and popular dairy dessert, but owing to its characteristics and valuable ingredients, it may be contaminated by many pathogenic and spoilage microorganisms. So, this study aimed to improve the quality and safety of MRP by using cinnamon and clove essential oils. Concerning the evaluation of the minimum inhibitory concentration (MIC) for both oils with 0.2, 0.5, and 1% concentrations, B. cereus and B. subtilis were sensitive (+) to cinnamon and clove 0.5%, with inhibition zones of 13.3 and 14 mm for cinnamon and 11.3 and 12 mm for clove EO, respectively. While both bacteria were very sensitive (++) to cinnamon 1% (18.8 and 19.5 mm) and clove 1% (17.3 and 18.7 mm), respectively. Therefore, MRP was prepared by adding cinnamon and clove EOs at 0.6%. Treatments containing EOs showed a significant reduction of tested microorganisms compared to controls. B. cereus wasn’t detected in clove and cinnamon EO treatments at day 21 of the storage period, while B. subtilis vanished on day 14 for the cinnamon treatment and on day 21 for clove MRP. Moreover, the results revealed the enhancement of sensory characteristics of MRP supplemented with EOs without any significant alteration in their pH values. This study recommends the addition of cinnamon and clove EOs (0.6%) to MRP, as it isn’t only an excellent substitution of chemical preservatives with powerful antibacterial efficiency but also improves the overall acceptance of the product.
Keywords
B. cereus; B. subtilis; Cinnamon; Clove; Milk Rice Pudding
References
ABD EL-WAHAAB, A., SAAD, S. M., HASSAN, M. A., and MAAROUF, A. A., 2018. Control of Bacillus Cereus in minced meat using some essential oils.Benha Veterinary Medical Journal, 34(3): 167-171. https://dx.doi.org/10.21608/bvmj.2018.44356

ABDEL-LATIF, E. F., and SAAD, M. F., 2016. Effect of bifidobacterium lactis on quality of rice pudding as a probiotic food carrier. International Journal of Current Microbiology and Applied Sciences, 5(8): 362-371. http://dx.doi.org/10.20546/ijcmas.2016.508.038

ADAM, A.H., SALWA, A.A. and SAAD, M.F., 2021. Evaluation of microbial quality and safety of selected dairy products with special focus on toxigenic genes of Bacillus cereus. Mljekarstvo, 71(4), pp.257-268. https://doi.org/10.15567/mljekarstvo.2021.0405

ADAM, A.H., SALWA, A.A. and SAAD, M.F., 2024. Microbiological quality of some commercial dairy products and cheese analogues focused on Mideast. Journal of the Hellenic Veterinary Medical Society, 75(1), pp.6945-6958.‏ https://doi.org/10.12681/jhvms.33549

AHMED, L. I., IBRAHIM, N., ABDEL-SALAM, A. B., and FAHIM, K. M., 2021. Potential application of ginger, clove and thyme essential oils to improve soft cheese microbial safety and sensory characteristics. Food Bioscience, 42: 101177.‏ https://doi.org/10.1016/j.fbio.2021.101177

ANTIGO, J., CESTARI, L., SCAPIM, M., DOS SANTOS, S.S., MORITZ, C.F. and MADRONA, G.S., 2017. Clove and cinnamon essential oils in dulce de leche. Nutrition and Food Science, 47(1), pp.101-107. http://dx.doi.org/10.1108/NFS-03-2016-0036

AOAC (Association of Official Analytical Chemistry), 2000. Official Methods of Analysis International, 17th Ed. AOAC, Washington, DC.

ATEF, N.M., SHANAB, S.M., NEGM, S.I., and ABBAS, Y.A., 2019. Evaluation of antimicrobial activity of some plant extracts against antibiotic susceptible and resistant bacterial strains causing wound infection. Bull. Natl. Res. Cent. https://doi.org/10.1186/s42269-019-0184-9

BABU, A. J., RUPASUNDARI, A., INDUMATHI, J., SRUJAN, R. V. N., and SRAVANTHI, M., 2011. Study on the antimicrobial activity and minimum inhibitory concentration of essential oils of spices. Veterinary World, 4(7), 311-316. http://dx.doi.org/10.5455/vetworld.2011.311-316

BAKR, J. G., KHALID, S. A., KHAFAGA, N. I., YASSIEN, N. A., and ZAKI, H. M., 2024. Impact of using cinnamon (Cinnamomum verum) essential oil and its pectin-chitosan nano-emulsion on survival of Aspergillus flavus and total aflatoxin inhibition in beef burger patties. Food Control, 159: 110294.‏ https://doi.org/10.1016/j.foodcont.2024.110294

BENNETT RW, TALLENT SM, and HAIT JM, 2015.  Bacillus cereus. In: Salinger, Y., Tortorello, M.L. (eds) Compendium of Methods for the Microbiological Examination of Foods, 5th edition. American Public Health Association, Washington, DC, USA. Chapter 31: 375–390.

CETIN-KARACA, H., and MORGAN, M. C., 2018. Inactivation of Bacillus cereus spores in infant formula by combination of high pressure and trans-cinnamaldehyde. LWT, 97: 254-260.‏ https://doi.org/10.1016/j.lwt.2018.07.001

CHANDRA, H., BISHNOI, P., YADAV, A., PATNI, B., MISHRA, A.P., and NAUTIYAL, A.R., 2017. Antimicrobial resistance and alternative resources with special emphasis on plant-based antimicrobials– A review. Plants 6 (2): 457–462. https://doi.org/10.3390/plants6020016

CHON, J.W., SEO, K.H., BAE, D., KIM, B., JEONG, D. and SONG, K.Y., 2020. Antibacterial activity of clove oil against foodborne pathogenic bacteria and sensory attributes in clove oil-enriched dairy products: a preliminary study. Journal of Dairy Science and Biotechnology, 38(4), pp.197-206. https://doi.org/10.22424/jdsb.2020.38.4.197

CHOOBKAR, N., DARAEI GARMAKHANY, A., AGHAJANI, A. R., and ATAEE, M., 2022. Response surface optimization of pudding formulation containing fish gelatin and clove (Syzygium aromaticum) and cinnamon (Cinnamomum verum) powder: Effect on color, physicochemical, and sensory attributes of the final pudding product. Food Science and Nutrition, 10(4):1257-1274.‏ https://doi.org/10.1002/fsn3.2761

CLSI (Clinical and Laboratory Standards Institute), 2018. Performance Standards for Antimicrobial Susceptibility Testing. M100S, twenty-eight Ed.

ELAFIFY, M., ALSAYEQH, A.F., ALJASIR, S.F., TAHON, A.B.M.B., ALY, S., SAAD, M. F, MOHAMED, E.A.A., DARWISH, W.S. and ABDELLATIF, S. S., 2023. Occurrence and characterization of toxigenic Bacillus cereus in dairy products with an inactivation trial using D-Tryptophan and ascorbic acid in the rice pudding. LWT, 175: 114485.‏ https://doi.org/10.1016/j.lwt.2023.114485

ELHENNAWY, R. A., MOAWAD, A.A., IBRAHIM, A.S., and HALAWA, M. A., 2023. Assessment of Selected Varieties of Cheese Analogues sold in Egyptian Markets. Egyptian Journal of Chemistry, 66(10), pp.509-520. https://dx.doi.org/10.21608/ejchem.2023.183559.7388

EL-KHOLY, A. M., MESHREF, A.M.S. and MORSY, B.A.M., 2022. Monitoring of Bacillus cereus in rice pudding. New Valley Veterinary Journal, 2(2), 53-57.‏ https://doi.org/10.21608/nvvj.2022.163521.1012

ELSHERIF, W. M, EL HENDY, A. H.M., ELNISR, N. A. and WAHBA, N. M., 2021. Studying the effect of chitosan on Bacillus cereus producing cereulide toxin in milk and some dairy desserts. Journal of microbiology, biotechnology and food sciences, 10(5): e3417-e3417.‏ https://doi.org/10.15414/jmbfs.3417

ELUMALAI, S., KESAVAN, R., RAMGANESH, S., PRAKASAM, V., and MURUGASEN, R., 2010. Comparative study on anti-microbial activities of bark oil extract from cinnamomum cassia and cinnamomum zeylanicum. Biosciences Biotechnology Research Asia, 7(1): 251-258.

FAGERE, Z. O., and Al MAGBOU, A. Z., 2016. Antibacterial activity of clove oil against some microorganisms at Khartoum state, Sudan. Advancement in Medicinal Plant Research, 4(4), 122-128.‏

FOOD AND DRUG ADMINISTRATION (FDA)., 2016. Substances generally recognized as safe. Eletronic code of federal regulations 21 cfr 182. U.S. Government Publishing Office.

Gupta, C., Garg, A. P., Uniyal, R. C., and Kumari, A., 2008. Antimicrobial activity of some herbal oils against common food-borne pathogens. African journal of microbiology research, 2(10): 258-261. https://doi.org/10.5897/AJMR.9000072

HAMMAD, A. M., 2016. Antimicrobial effect of cinnamon and clove on Staphylococcus aureus in milk and yogurt. Alexandria Journal of Veterinary Sciences, 48(1). http://dx.doi.org/10.5455/ajvs.203629

HETTA, H. F., KH MESHAAL, A., ALGAMMAL, A. M., YAHIA, R., MAKHARITA, R. R., MARRAIKI, N., SHAH, M.A., HASSAN, H.M. and BATIHA, G. E., 2020. In-vitro antimicrobial activity of essential oils and spices powder of some medicinal plants against bacillus species isolated from raw and processed meat. Infection and Drug Resistance, 13: 4367-4378.‏ https://doi.org/10.2147/idr.s277295

HUSSEIN, A., POSSAS, A., HASSANIEN, A. A., SHAKER, E. M., and VALERO, A., 2023. Assessing Listeria monocytogenes growth kinetics in rice pudding at different storage temperatures. International Journal of Food Microbiology, 404: 110346.‏ https://doi.org/10.1016/j.ijfoodmicro.2023.110346

IBRAHEIM, H.I.F., ABDELKHALEK, A. and ELSHERBINI, M., 2023. Prevalence of Bacillus Cereus Virulence Genes in Some Dairy Desserts. Mansoura Veterinary Medical Journal, 24(3), 13.‏  https://doi.org/10.35943/2682-2512.1004

IBRAHIM, A.S., ZAHRAN, H., AWAAD, S.S. and HEGAB, O.W., 2023. Comparative evaluation of fatty acid profiles and lipid nutritional indexes in Egyptian fresh cow, buffalo, goat soft cheeses and their mixtures. Egyptian Journal of Chemistry, 66(11), pp.415-424. https://doi.org/10.21608/ejchem.2023.206528.7879

JAVED S, MUSHTAQ S, KHOKHAR I, AHMAD R., and HAIDER MS, 2012. Comparative antimicrobial activity of clove and fennel essential oils against food borne pathogenic fungi and food spoilage bacteria. Afr J Biotechnol, 11(94): 16065-16070. https://doi.org/10.5897/AJB11.3058

KWON J. A., Yu C. B., and PARK H. D. 2003. Bacteriocidal effect and inhibition of cell separation of cinnamic aldehyde on Bacillus cereus. Letters of Applied Microbiology, 37(1): 61-65. https://doi.org/10.1046/j.1472-765x.2003.01350.x

LAWLESS, H.T. and HEYMANN, H., 2010. Sensory evaluation of food: principles and practices. Springer Science and Business Media.

LIANOU, A., MOSCHONAS, G., NYCHAS, G. J. E., and PANAGOU, E. Z., 2018. Growth of Listeria monocytogenes in pasteurized vanilla cream pudding as affected by storage temperature and the presence of cinnamon extract. Food Research International, 106: 1114-1122.‏ https://doi.org/10.1016/j.foodres.2017.11.027

MOREIRA, M. R., PONCE, A. G., DE VALLE, C. E., and ROURA, S. I., 2005. Inhibitory parameters of essential oils to reduce a foodborne pathogen. LWT, 38, 565–570.  https://doi.org/10.1016/j.lwt.2004.07.012

MOSCHONAS, G., LIANOU, A., NYCHAS, G. J. E., and PANAGOU, E. Z., 2021. Spoilage potential of Bacillus subtilis in a neutral-pH dairy dessert. Food Microbiology, 95: 103715.‏ https://doi.org/10.1016/j.fm.2020.103715

NASROLLAHZADEH, A., MOKHTARI, S., KHOMEIRI, M. and SARIS, P.E., 2022. Antifungal preservation of food by lactic acid bacteria. Foods, 11(3), p.395. https://doi.org/10.3390/foods11030395

OMAR, A.A.A.H., GAD, M.F., ABDELHAFEZ, H.M., EBRAHEM MERSAL, A.T. and MOSSA, A.T.H., 2023.Phytochemical Study, Antioxidant Potential and Preparation of a Clove Nanoemulsion Loaded with Pomegranate Peel Extract. Egyptian Journal of Chemistry, 66(13): 21-37. https://doi.org/10.21608/ejchem.2023.213866.8034

OZCAN, T., YILMAZ-ERSAN, L., AKPINAR-BAYIZIT, A., SAHIN, O. I., and AYDINOL, P., 2010. Viability of Lactobacillus acidophilus LA-5 and Bifidobacterium bifidum BB-12 in Rice Pudding. Mljekarstvo, 60(2): 135-144.‏

PONCE, A. G., FRITZ, R., DEL VALLE, C. E., and ROURA, S. I., 2003. Antimicrobial activity of essential oils on the native microflora of organic Swiss chard. LWT, 36, 679–684. https://doi.org/10.1016/S0023-6438(03)00088-4

PRABUSEENIVASAN, S., JAYAKUMAR, M., and IGNACIMUTHU, S., 2006. In vitro antibacterial activity of some plant essential oils. BMC complementary and alternative medicine, 6: 1-8. https://doi.org/10.1186/1472-6882-6-39

RAHNAMA, H., AZARI, R., YOUSEFI, M. H., BERIZI, E., MAZLOOMI, S. M., HOSSEINZADEH, S., DERAKHSHAN, Z., FERRANTE, M. and CONTI, G. O., 2023. A systematic review and meta-analysis of the prevalence of Bacillus cereus in foods. Food Control, 143: 109250.‏ https://doi.org/10.1016/j.foodcont.2022.109250

SALIH, G. M., JASSIM, M. A. and SAADI, A. M. 2021. Effect cardamom and cinnamon supplemented with soft cheese on tissues rats. Food Research, 5(1), 211-215.‏ https://doi.org/10.26656/fr.2017.5(1).501

SALEH, A. E., 2018. Keeping Quality of Kareish Chees as Affected with Added Certein Spices and Herbs Oil as Antifungal Agents. Journal of Food and Dairy Sciences, 9(1): 7-12. https://dx.doi.org/10.21608/jfds.2018.35145

‏‏SALMAN, K. H., MAHMOUD, E. A., and Abd-ALLAB, A. E. A., 2020. Utilization of Cinnamon, Clove and Thyme Essential Oils as Antimicrobial and Bioactive Compounds in Kishk Manufacturing. Suez Canal University Journal of Food Sciences, 7(1): 43-54. https://dx.doi.org/10.21608/scuj.2020.130639

SETHUNGA, M., RANASINGHE, M. M. K. D., RANAWEERA, K. K. D. S., MUNAWEERA, I., and GUNATHILAKE, K. D. P. P., 2023. Synergistic antimicrobial activity of essential oils and oleoresins of cinnamon (Cinnamomum zeylanicum), clove bud (Syzygium aromaticum) and ginger (Zingiber officinale). Biocatalysis and Agricultural Biotechnology, 51: 102800.‏ https://doi.org/10.1016/j.bcab.2023.102800

SHAWKI, M.A., ABDEL-LATIF, E.F., DARWISH, S.F., SAUDI, A.M. and ALI, Z.I., 2024. Assessment of the Chemical and Microbiological Quality of Some Cheese Assortment in Egypt: Highlighting the incidence of multidrug-resistant Staphylococcus Species. Journal of Applied Veterinary Sciences, 9(1), pp.85-93. https://dx.doi.org/10.21608/javs.2023.243014.1285

SIDDIQUA, S., ANUSHA, B. A., ASHWINI, L. S., and NEGI, P. S., 2015. Antibacterial activity of cinnamaldehyde and clove oil: effect on selected foodborne pathogens in model food systems and watermelon juice. Journal of food science and technology, 52: 5834-5841. https://doi.org/10.1007%2Fs13197-014-1642-x

SPSS, 2017.Version 25. Armonk, NY: IBM Crop.

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