Evaluation of the Role of Carvacrol Against Acetamiprid Induced Apoptosis and DNA Damage in Liver and Kidney of Adult Male Albino Rats | ||
| Zagazig Journal of Forensic Medicine and Toxicology | ||
| Article 2, Volume 22, Issue 2, July 2024, Pages 17-52 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/zjfm.2024.269995.1177 | ||
| Authors | ||
| Nahla Mohamed Ibrahim1; Wesam Barakat Assy* 2; Samar R. Mohamed3; Samaa M El-Mahroky4; Hend Sameh5; Elham Elshawadfy Megahed6 | ||
| 1Forensic Medicine and clinical toxicology department, faculty of medicine,zagazig university | ||
| 2Forensic medicine and clinical Toxicology department, faculty of Medicine, Zagazig university. | ||
| 3Medical Histology and Cell Biology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt | ||
| 4Histology and cell biology department, faculty of medicine, Zagazig university, Zagazig, Egypt. | ||
| 5Biochemistry department, faculty of medicine, Zagazig university, Zagazig, Egypt. | ||
| 6forensic medicine and toxicology, medicine faculty, zagazig university | ||
| Abstract | ||
| Human Exposure to acetamiprid (ACP) is highly frequent because it is widely used insecticide. Acetamiprid (ACP) has potential toxic health effects on liver and kidney that make it an issue of concern to public health. Carvacrol is an antioxidant and has therapeutic uses. Aim: This work was done to evaluate the Carvacrol protective role against highly toxic effects of acetamipride produced biochemical, histo-pathological and immune-histochemical changes in renal and liver tissues of male albino rats. Methods: Thirty two adult male albino rats were equally divided into 4 groups: Group I (a) (Negative control group), Group I (b) (carvacrol control group) Group ІІ: acetampride group, Group ІIІ (Acetamprid-carvacrol group). At the end of the 4th week, 24 hours after the last dose, serum ALT, AST, Total bilirubin, Urea, uric acid and Creatinine. Hepatic and renal tissue MDA, catalase and reduced glutathione were measured. DNA fragmentation assay was done and DNA fragmentation visualized on gel electrophoresis in both hepatic and renal tissues. Histopathological study by H&E and Mallory trachome stain was done. Immunohistochemical staining by cleaved caspase 3 and iNOS were demonstrated. Results: In the acetamparide treated rats, there was increase in serum ALT, AST, total bilirubin, uric acid, Creatinine and Urea levels. Also, acetamparide induced elevation in hepatic and renal tissues MDA. Acetamparide caused decrease in hepatic and renal tissue catalase and GSH. The DNA fragmentation assay detected increased DNA fragmentation in both hepatic and renal tissues of the acetamparide group and was lowered by Co-administration of carvacrol. Histopathology and Immunohistochemical staining showed that: Acetamparide induced histological damages and strong immunoreaction decreased by co-treatment of carvacrol. Conclusion: Administration of carvacrol produced partial and incomplete improvement of liver and kidney function and histology beside an improvement in oxidative stress and inflammation caused by acetamparide. | ||
| Keywords | ||
| carvacrol; acetamparide; hepatotoxicity; nephrotoxicity; rats | ||
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