Identification of A 38 KDa Antigen in the Urine of Schistosoma Mansoni Chronicaily Infected Patients Using A Specific Antibody Microeluted from A Nitrocellulose Membrane; A Diagnostic Tool | ||
| Bulletin of Egyptian Society for Physiological Sciences | ||
| Article 9, Volume 30, Issue 1, December 2010, Pages 149-158 PDF (183.83 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/besps.2010.36170 | ||
| Author | ||
| Samir Mahgoub* | ||
| Department of Biochemistry, Faculty of Medicine, Al-Minia University | ||
| Abstract | ||
| Schistosomiasis is a chronic debilitating disease affecting 200 -300 million throughout the world, a major focus of research has been done to identify and characterize antigens that may have vaccine and/or diagnostic potential. In the present study, NP-40 extracted surface proteins of Schistosoma mansoni (S. mansoni) adult worms were subjected to 12.5% Sodium Dodecyl Sulfate– Polyacrylamide Gel Electrophoresis (SDS-PAGE) and electrotransfered onto a nitrocellulose membrane (PVDF), then, incubated with pooled sera collected from S. mansoni chronically infected patients. The presence of antibodies identify a number of NP-40 extracted surface proteins of the adult worms of S. mansoni. One of these proteins of 38 kDa molecular weight with high immunogenicity was selected. The strip of nitrocellulose membrane containing the complex of the identified protein and its specific antibody was cut guided by the molecular weight marker, then, the antibody was micro-eluted. Proteins of urine samples from the same patients were precipitated and purified over G-Sephadex column. The purified proteins of urine samples and proteins of sera were subjected to another SDS- PAGE and electrotransferred onto PVDF membrane. The microeluted antibody was used to identify an antigen of 38 kDa molecular weight in sera of the chronically infected patients as well as in urine. The 38 kDa antigen was excreted in the urine of those patients in a stable form and detected by the specific monoclonal antibody. The active epitope of 38 kDa antigen could be a promising immunochemical probe for S. mansoni infection diagnosis. Further studies will be done to characterize that antigen as well as its potential application in immunodiagnosis for S. mansoni | ||
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