Isolation and Diagnosis of Some Types of Fungi Using PCR Technology for Infection by (Musca domestica L.) From some Areas of Anbar Governorate | ||
| Journal of Bioscience and Applied Research | ||
| Volume 10, Issue 2, June 2024, Pages 276-289 PDF (2.16 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jbaar.2024.285734.1045 | ||
| Authors | ||
| Saffanah Mushrif Hardan* 1; Thamer A. A. Muhsen2 | ||
| 1Anbar | ||
| 2Dept of Biology, College of Education for pure Science (Ibn Al-Haitham), University of Baghdad, Iraq. | ||
| Abstract | ||
| This study revealed the presence of several fungal species, some of which are saprophytic, and others are pathogenic, in addition to the presence of fungi used in biocontrol in soil samples collected from six different regions of Anbar province. The identified fungal genera include Metarhizium anisopliae, Trichoderma harzianum, Penicillium, Fusarium spp., Rhodotorula, Rhizopus, Candida spp., and Aspergillus flavus. Confirmation of the diagnosis of the pathogen of the housefly (Musca domestica) using PCR technique indicated the presence of different genera of fungi at various concentrations, with the highest frequency observed for Aspergillus flavus (78.2%) and the lowest frequency for Trichoderma harzianum (6.5%). Among the isolated fungi, pathogenic fungi belonging to the genus Metarhizium anisopliae and Trichoderma harzianum were identified. Molecular diagnosis of the two pathogenic fungi was conducted using the polymerase chain reaction (PCR) technique, and the purity of the isolates ranged between 1.69 and 1.94, with concentrations ranging from 34.03 to 52.10 ng/ml. The newly isolated strains were deposited in the NCBI Gene Bank under the researchers' names. | ||
| Keywords | ||
| Diagnosis of some types of fungi; PCR molecular diagnostic technique; Trichoderma harzianum; Metarhizium anisopliae | ||
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