Multiplication and Control of vitrification during culture establishment of Arganiaspinosa L. | ||
| Horticulture Research Journal | ||
| Volume 2, Issue 1, June 2024, Pages 1-13 PDF (603.07 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/hrj.2024.361887 | ||
| Abstract | ||
| The aim of this study was to reach a well-defined protocol to in vitro propagate of Arganiaspinosaand overcome vitrification phenomenon. In this respect, the highest number of shoot was found at 2.0 mg/l Kin at genotype 3 for multiplication stage. MS medium supplemented with 100 mg/l calcium pantothenate was suited for to eliminate vitrification at all genotypes. For callus induction, MS medium supplemented with 1.5 mg/l NAA when incubated in the light or darkness was favorable to callus induction. MS medium supplemented with 0.5 mg/l GA3 + 1.5 mg/l BA led to increases shoot initiation. | ||
| Keywords | ||
| Micropropagation- In vitro - Tissue culture; Argan; Callus induction | ||
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