Association Between Polymorphism of Myf5 Gene And Growth Traits In Bovine | ||
| Veterinary Medical Journal (Giza) | ||
| Volume 62, Issue 1, January 2016, Pages 53-58 PDF (2.29 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/vmjg.2016.363273 | ||
| Authors | ||
| Els Awad* ; S Abdelaziz; M Warda; I Ahmed | ||
| Dept. of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, Cairo University | ||
| Abstract | ||
| Carcass quantity is largely affected by growth rate which is one of the most economically important quantitative traits. MYF5, a member of the myogenic regulatory factors, was chosen as candidate gene for growth traits due to its important role in growth and development of mammals. The objectives of this study were to determine the single nucleotide polymorphism (SNP) in MYFS5 gene, estimate the allele and genotype frequencies of the MYFS gene and to determine the effect of this polymorphism on some growth traits in cattle and buffalo. Genomic DNA was extracted from whole blood of 100 Friesian cattle and 100 Egyptian buffalo. The PCR products of MYFS gene were subjected to genotyping. PCR-SSCP technique was used to genotype MY FS gene at the intron-1 locus. Only one genotype (AB) was detected in cattle and so no association analysis was performed. While in buffalo two genotypes were determined (AC and BB). Significant positive association was found between BB genotype and weight at 6 months and average daily gain. However, no significant association between the genotypes and birth weight and weaning weight was observed. Sequencing results revealed a transition of A > G at position 1385 in intron-1 of the MYF5 gene in buffalo. These results could be included into marker assisted selection programs to improve the productivity in these buffalo breed. | ||
| Keywords | ||
| myogenic regulatory factors; MYF5; genotyping; growth traits; buffalo; cattle | ||
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