Kandeel, S., Remon S. Estfanous, R., Badr, S. (2024). The possible protective effect of pumpkin seed oil on dutasteride-induced changes on spleen of adult male albino rats: Histological and immunohistochemical study. test, 2(2), 52-81. doi: 10.21608/ejctr.2024.286991.1016
Samah Kandeel; Remon Remon S. Estfanous; Shimaa Mahmoud Badr. "The possible protective effect of pumpkin seed oil on dutasteride-induced changes on spleen of adult male albino rats: Histological and immunohistochemical study". test, 2, 2, 2024, 52-81. doi: 10.21608/ejctr.2024.286991.1016
Kandeel, S., Remon S. Estfanous, R., Badr, S. (2024). 'The possible protective effect of pumpkin seed oil on dutasteride-induced changes on spleen of adult male albino rats: Histological and immunohistochemical study', test, 2(2), pp. 52-81. doi: 10.21608/ejctr.2024.286991.1016
Kandeel, S., Remon S. Estfanous, R., Badr, S. The possible protective effect of pumpkin seed oil on dutasteride-induced changes on spleen of adult male albino rats: Histological and immunohistochemical study. test, 2024; 2(2): 52-81. doi: 10.21608/ejctr.2024.286991.1016

The possible protective effect of pumpkin seed oil on dutasteride-induced changes on spleen of adult male albino rats: Histological and immunohistochemical study

Egyptian Journal of Cell and Tissue Research
Volume 2, Issue 2, June 2024, Pages 52-81    PDF (1.09 M)
Document Type: Original articles
DOI: 10.21608/ejctr.2024.286991.1016
Authors
Samah Kandeel* 1; Remon Remon S. Estfanous2; Shimaa Mahmoud Badr3
1Professor of Histology and Cell Biology Faculty of Medicine, Tanta University
2Anatomy and Embryology department, Faculty of Medicine, Tanta University, Egypt..
3Histology &cell biology Department _Faculty of Medicine _Tanta University
Abstract
Background: Dutasteride is an anti-androgen used in the treatment of prostatic diseases. Pumpkin seed oil (PSO) is a functional food oil with an antioxidant and immunomodulatory actions. Aim: assessing the possible protective effect of PSO on dutasteride-induced changes on spleen of adult male albino rats via histological and immunohistochemical methods. Methods: 30 male albino rats 200-250 gm divided into; Control group, Dutasteride low dose (DLD): rats received dutasteride 0.05 ml /day, Dutasteride high dose (DHD): rats received dutasteride 0.15 ml/ day, DLD + PSO: dutasteride low dose group treated with 1.5 ml/kg/day of PSO, DHD + PSO: dutasteride high dose treated with 1.5 ml/kg/day of PSO. Doses were orally for 42 days. Results: DLD &DHD groups (Groups II &III) showed marked histopathological changes dose dependently. There was dilated blood sinusoids in the subcapsular areas & throughout the parenchyma, disorganization, dilatation& expansion of the red pulp with reduced splenic cords, disorganization & reduction in the size of the white pulp lymphoid follicles having lymphocytes with pyknotic nuclei and vacuolated cytoplasm, macrophages containing apoptotic bodies, dilated central arteriole with detached endothelium and vacuolated wall, and significantly increased collagen mean area%. Also, significantly increased mean number of immune-positive cells for CD68& TGF-β while decreased for CD3 immunohistochemical expression. Regarding the DLD+PSO& DHD+PSO groups (Groups III& IV); marked amelioration of the previously mentioned results was observed. Conclusion: PSO has an ameliorative effect on the histopathological changes on rat spleen induced by dutasteride. So, PSO is beneficial in the treatment of side effects of dutasteride.
Keywords
Spleen; Dutasteride; Pumpkin Seed Oil; Histopathology
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