Role of Advanced Glycation End Products and Vascular Endothelial Growth Factor in Pathophysiology of Diabetic Retinopathy | ||
| Bulletin of Egyptian Society for Physiological Sciences | ||
| Article 12, Volume 29, Issue 1, June 2009, Pages 137-148 PDF (217.29 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/besps.2009.36334 | ||
| Authors | ||
| Marwa Ahmed* 1; Omar Aly2; Ehab Wasfy2; Salwa Wasfy1 | ||
| 1Physiology Department, Faculty of Medicine, Assiut University | ||
| 2Ophthalmology Department, Faculty of Medicine, Assiut University | ||
| Abstract | ||
| Background: The hyperglycemic state occurring in diabetes mellitus is responsible for formation and accumulation of advanced glycation end products (AGEs) that participate with their receptors, receptor for advanced glycation end products (RAGE ) in the pathogenesis of vascular complications of diabetes mellitus. Intraocular vascular endothelial growth factor (VEGF) levels have been studied in animal models and human vitreous fluid, where the levels are found to be high in patients with active intraocular neovascularization. Objective: To investigate the levels of AGEs ,soluble form of RAGE (sRAGE) ,VEGF and total antioxidants (TAO) in both vitreous and blood of diabetic patients with and without diabetic retinopathy. Study Design: Blood and vitreous samples were collected from 30 diabetic patients, 15 with proliferative diabetic retinopathy (PDR), 15 without retinopathy, and 15 non diabetic control subjects. ELISA technique was used for measuring vitreous and blood levels of AGE, sRAGE, VEGF and TAO. Results: AGEs, sRAGE and VEGF levels in blood were significantly higher in all diabetic groups compared to controls and in PDR patients compared to diabetic group. There were positive correlations between serum AGEs, sRAGE and VEGF levels in both diabetic groups. Vitreous levels of AGEs and VEGF were significantly increased in all diabetic groups compared to controls and in PDR group compared to diabetic group. Also there were significant correlations between these levels in both PDR and diabetic groups. Serum and vitreous TAO levels were decreased in patients with diabetes compared to controls and in patients with PDR compared to diabetics without retinopathy. Furthermore, Serum TAO levels were inversely correlated with serum levels of AGEs, sRAGE and VEGF in all diabetic patients. TAO levels in vitreous were inversely correlated with vitreous levels of AGEs, and VEGF in all diabetic patients. Conclusion: These findings suggest that the interaction of advanced glycation end products (AGEs), with their cellular receptor (RAGE) and oxidative stress is implicated in the pathogenesis of diabetic vascular complications through stimulation of VEGF. | ||
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