SOME VIROLOGICAL AND PATHOLOGICAL STUDIES ON CAMEL PDX | ||
| Egyptian Journal of Agricultural Research | ||
| Article 26, Volume 76, Issue 4, December 1998, Pages 1671-1688 PDF (6.14 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejar.1998.363520 | ||
| Authors | ||
| MAYSA H. SHAKER1; MOHAMED H. AHMED1; SAYED A. SALEM1; MAHMOUD M. ELKABANY2 | ||
| 1Animal Health Research Institute, Agricultural Research Centre, Dokki, Giza, Egypt | ||
| 2Serum and Vaccine Research Institute, Agricultural Research Centre, Giza, Egypt | ||
| Abstract | ||
| In private camels farm at Marsamatroh Governorate, Egypt, 5 out of 100 dromedary camels showed clinical manifestation referred to camel pox. The lesions were confined to the skin of the head, nose, eyelids and neck. Five from skin biopsies were taken for virological and pathological studies. The characterization of the viral isolate revealed its multiplication on the corioallantoic membrane (CAM) of chicken embryos at the age of 11 days with the formation of minute white pock lesions 5 days post-inoculation_ Histopathological investigation showed eosinophilic cytoplasmic inclusion bodies in the cells of CAM. The virus was identified by agar gel immunodiffusion test using specific hyperinnmune serum of camel pox virus. Electron microscopy was done on two skin biopsies and one sample from CAM, as well as 3 other CAM samples for negative stain. Pox virions were detected in the cytoplasm of CAM cells and in the cells of tunica intima of dermal blood vessels. Histopathological examination of skin biopsies showed degeneration and necrosis of prickle cells, as well as, vascular deamage in the dermis with mononuclear cell infiltration around the blood vessels. Intracytoplamic inclusion bodies were seen in some prickle cells which appeared as homogenous round eosinophilic bodies. | ||
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