Short Communication Notes on the role of Echis coloratus and Naja nigricollis Snake venoms on neuronal cell death | ||
| Bulletin of Egyptian Society for Physiological Sciences | ||
| Article 6, Volume 29, Issue 2, December 2009, Pages 83-94 PDF (212.82 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/besps.2009.36354 | ||
| Author | ||
| Ahmed Abdel.Zaher* | ||
| Zagazig University - Faculty of Science – Department of Chemistry- Biochemistry division King Saud University- College of science and Arts in Shagra- Kingdom of Saudi Arabia | ||
| Abstract | ||
| In the present study murine hippocampal HT22 cells were employed to investigate the role of Echis coloratus and Naja nigricollis snake venom on cell death. Monitoring of the release of the cytoplasmic enzyme lactate dehydrogenase (LDH) in the culture medium after treatment of the cells with different concentrations (50ug/ml, 100 μg/ml) from four fractions (F1, F2, F3 and F4) that obtained from each venom after purification. The time variation (6, 12, 18 and 24 hours) of the LDH concentration in the medium was used to indicate the total amount of lysed and, hence, the specific rates of cell death. In the first 6h from treatment of cells with F2( 50 μg/ml ) from Naja nigricollis venom( which is the most effective fraction in both venoms), LDH released into cell culture media more than treatment of cells with F3 and crude venom. Treatment of cells with a concentration of 50ug/ml and 100 μg/ml F3 Naja nigricollis (after 12h and 24h) snake venom, LDH elevated more than F2 and crude venom. Otherwise, lactate dehydrogenase (LDH) was released into cell culture media by treatment of the cell by 50 μg/ml F3 more than treatment of cells by F4 and crude venom from Echis coloratus. | ||
| Keywords | ||
| Echis coloratus; Naja nigricollis; HT22 cells- LDH | ||
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