Corn Silk (Stigma maydis) Ameliorates Indomethacin–Induced Colitis in Rats via Modulation of Keap1/Nrf2 Signaling Pathway | ||
| Egyptian Journal of Veterinary Sciences | ||
| Articles in Press, Accepted Manuscript, Available Online from 01 June 2025 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejvs.2024.283915.2020 | ||
| Authors | ||
| Hadeer Aboumosalem1; Abd-Allah A. Mokhbatly2; Wael M. Goda3; Emad W. Ghazy4; Mohamed F. Abou Elazab5; Amany Ragab6; Alaa Abdelatty7; Zizy Ibrahim Elbialy* 8; Doaa Hosny Assar9 | ||
| 11Clinical Pathology Department, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh 33516, Egypt. | ||
| 21Clinical Pathology Department, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh 33516, Egypt | ||
| 3Faculty of Veterinary Medicine, Department of Clinical Pathology, Damanhour University, Egypt | ||
| 4Clinical Pathology Department, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh 33516, Egypt. | ||
| 5Clinical Pathology Department, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh 33516, Egypt | ||
| 6Pharmacognosy Department, Faculty of Pharmacy, Tanta University, Tanta 31527, Egypt | ||
| 7Pathology Department, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh 33516, Egypt | ||
| 833516 Al Geish street, Kafrelsheikh University, | ||
| 9Clinical Pathology Department, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh 33516, Egypt33516 Al Geish Street, Kafrelsheikh University, | ||
| Abstract | ||
| As a public health problem, ulcerative colitis (UC) is a common inflammatory bowel disease associated with inflammatory perturbation and oxidative stress. Our work investigated the anti-colitic effects of corn silk (Stigma maydis) on indomethacin-induced ulcerative colitis and further explored its potential mechanism. Moreover, phytochemical characterization of the corn silk aqueous methanol extract was analyzed by UPLC-ESI-MS/MS analysis to rationalize the results of the biological investigation. Adult male Wistar rats (60) were divided randomly into six groups for 21 days. 1st group (control negative), 2nd group indomethacin (control positive), 3rd group sulfasalazine (positive drug control) at a dose of 100 mg/kg b.w. They were given corn silk orally in a dose of 150 or 300 mg/kg bw /day for 14 days before and after induction of UC on the 15th and 16th day of treatment representing the 4th and 5th groups respectively. The 6th group received (corn silk at a dose 300 mg/kg b.w + sulfasalazine. Our results revealed that corn silk could markedly lighten the adverse impacts of indomethacin by restoring the leukogram to normal, reducing DAI, colon weight, macroscopic damage, colonic MDA and NO levels, Caspase-3 and expressions levels of keap1 and significantly reduced histopathological deterioration. Additionally, it significantly enhanced colon length, Catalase activity and GSH level with elevation of Nrf2 gene expression level compared to control positive group. Overall, the effect of corn silk for alleviating colitis was largely mediated by modulating the signaling pathway of keap1-Nrf2 genes that is attributed to the phytochemical characterization of the phenolic (mainly flavonoids) and hydroxylated fatty acids components in corn silk extract that restored antioxidants and prevented inflammatory and apoptotic cell damages in rats. For that, corn silk holds a promising potential approach for lowering the risk of developing colitis. To the best of our understanding, this study marks the first instance of documenting the protective effect of corn silk against indomethacin-induced colitis. | ||
| Keywords | ||
| colitis; indomethacin; corn silk; UPLC-ESI-MS/MS; sulfasalazine; antioxidant; Gene expression; Rats | ||
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