Sharaf, S., Ali, H., Salah, M., Kamel, Z. (2024). Insight on the Prevalence of Clinical Klebsiella Isolates Producing Extended Spectrum Beta-Lactamases. test, 33(3), 125-131. doi: 10.21608/ejmm.2024.298504.1267
Sherin M.A. Sharaf; Hanaa Toson Ahmed Ali; Mohamed Gamal Salah; Zeinat Kamel. "Insight on the Prevalence of Clinical Klebsiella Isolates Producing Extended Spectrum Beta-Lactamases". test, 33, 3, 2024, 125-131. doi: 10.21608/ejmm.2024.298504.1267
Sharaf, S., Ali, H., Salah, M., Kamel, Z. (2024). 'Insight on the Prevalence of Clinical Klebsiella Isolates Producing Extended Spectrum Beta-Lactamases', test, 33(3), pp. 125-131. doi: 10.21608/ejmm.2024.298504.1267
Sharaf, S., Ali, H., Salah, M., Kamel, Z. Insight on the Prevalence of Clinical Klebsiella Isolates Producing Extended Spectrum Beta-Lactamases. test, 2024; 33(3): 125-131. doi: 10.21608/ejmm.2024.298504.1267

Insight on the Prevalence of Clinical Klebsiella Isolates Producing Extended Spectrum Beta-Lactamases

Egyptian Journal of Medical Microbiology
Volume 33, Issue 3, July 2024, Pages 125-131    PDF (413.77 K)
Document Type: New and original researches in the field of Microbiology.
DOI: 10.21608/ejmm.2024.298504.1267
Authors
Sherin M.A. Sharaf1; Hanaa Toson Ahmed Ali2; Mohamed Gamal Salah* 2; Zeinat Kamel2
1Department of Microbiology, Egyptian Drug Authority (EDA), Giza, Egypt
2Botany and Microbiology Department, Faculty of Science, Cairo University, 12613 Giza, Egypt
Abstract
Background: Antimicrobial resistance is increasing, particularly in Gram-negative bacteria such as Klebsiella species, which can cause acute infections and complications in intensive care units. Objectives: This study aims to investigate antimicrobial susceptibility patterns and the frequency of resistance among clinical Klebsiella isolates, focusing on prevalence of extended spectrum β-lactamases. Methodology: One hundred and eighty-one clinical isolates of Klebsiella spp. obtained from the Kasr Al Ainy Educational Hospital's Microbiological Laboratorywere subjected to phenotypic identification and antibiotics susceptibility testing using the Vitek 2 system. Results: 115 isolates out of 181 were identified as Klebsiella species. Of which, K. pneumoniae represented 89% while K. ozaenae and K. oxytoca were 7 and 4%, respectively. Only 53 isolates displayed extended spectrum β-lactamases (ESBL) Klebsiella. Throughout the susceptibility test of thirteen antibiotics, the highest resistant pattern (100%) was observed against ampicillin. Furthermore, ESBL Klebsiella spp. exhibited high resistance against cefotaxime, amikacin, and ciprofloxacin. Conclusion: This investigation revealed that K. pneumoniae subsp. pneumoniae was the dominant followed by K. pneumoniae subsp. ozaenae, and K. oxytoca. The highest resistance was identified among ESBL-producing clinical isolates; also, our results indicate extended drug resistance among non-ESBL-producing isolates.
Keywords
Enterobacteriaceae; ESBLs; Klebsiella species; antibiotic resistance
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