Role of stabilizers in preparation of a potent sheep pox vaccine | ||
| Veterinary Medical Journal (Giza) | ||
| Volume 58, Issue 1, January 2010, Pages 1-13 PDF (4.85 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/vmjg.2024.366946 | ||
| Authors | ||
| Christine Mikhael* 1; A El-sanousi2; I Reda2; S Rizkallah1 | ||
| 1Department of Pox, Veterinary Sera and Vaccines Research Institute, Abbassia, Cairo | ||
| 2Department of Virology, Faculty of Veterinary Medicine, Cairo University | ||
| Abstract | ||
| Production of live attenuated sheep pox vaccine sustained the elevated temperatures during freeze-drying, transportation storage and vaccination in unequipped tropical and subtropical zones of the world, is highly recommended. For this reason, eight stabilizer formulas were individually used for preparation of eight sheep pox vaccines, which were lyophilized and then titrated and accordingly four vaccine formulas were eventually selected that should be tested for thermo protectivity to select the best stabilized vaccine. These selected vaccines were tested for sterility; potency (vaccination and challenge) and safety in susceptible sheep. The collected blood sera were subject to serological examination for estimating the antibody response by ELISA. The results proved transcendence of sheep pox vaccines stabilized with 10% trehalose alone or in combination with 5% lactalbumin hydrolyste in the thermoprotectivey, thereby improvement vaccination efficacy. | ||
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