M, Y., A, H., M, E., A, E., Shalaby, M. (2011). Molecular detection, isolation and characterization of avian rotavirus from broiler chicken. test, 57(4), 573-584. doi: 10.21608/vmjg.2011.368032
Youssef M; Hussein A; El-sabagh M; El-Ibiary A; M Shalaby. "Molecular detection, isolation and characterization of avian rotavirus from broiler chicken". test, 57, 4, 2011, 573-584. doi: 10.21608/vmjg.2011.368032
M, Y., A, H., M, E., A, E., Shalaby, M. (2011). 'Molecular detection, isolation and characterization of avian rotavirus from broiler chicken', test, 57(4), pp. 573-584. doi: 10.21608/vmjg.2011.368032
M, Y., A, H., M, E., A, E., Shalaby, M. Molecular detection, isolation and characterization of avian rotavirus from broiler chicken. test, 2011; 57(4): 573-584. doi: 10.21608/vmjg.2011.368032

Molecular detection, isolation and characterization of avian rotavirus from broiler chicken

Veterinary Medical Journal (Giza)
Volume 57, Issue 4, October 2009, Pages 573-584    PDF (1.66 M)
Document Type: Original Article
DOI: 10.21608/vmjg.2011.368032
Authors
Youssef M1; Hussein A2; El-sabagh M2; El-Ibiary A1; M Shalaby3
1Central Laboratory of Quality control of Veterinary Biologics, Abbasia-Egypt.
2Department of Virology, Faculty of Veterinary Medicine, Cairo Univ., Giza Egypt.
3Department of Virology, Faculty of Veterinary Medicine, Cairo Univ., Giza Egypt
Abstract
Chicken rotaviruses (CRVs) were detected, identified and characterized in broiler chicken with diarrhea for the first time in Egypt. Feral samples were collected from 85 naturally occurring diarrheal outbreaks in commercial chicken broilers farms that are located in a wide range of geographical areas including many Egyptian governorates mainly 6th Octobar, EL-mansoura during year 2008. CRV was detected in the fecal samples by ELISA using monoclonal antibodies (Mabs) against VP6, Electron Microscopy (EM), Reverse Transcription Polymerase Chain Reaction (RT-PCR) and the virus was isolated using SPF chickens. The obtained results confirmed the isolation and identification of group A chicken rotavirus while the molecular characterization analysis using different primers sets suggested that the isolated chicken rotavirus does not belong to the same cluster of Ch1 rotavirus Strain but most likely more related to Po-13 strain (mammalian like chicken rotavirus strains). This study reports the importance of rotaviruses in broiler chickens with delayed growth and diarrhea.
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