Occurrence of Enterobacter sakazakii in some meat products sold in Assiut governorate | ||
| Veterinary Medical Journal (Giza) | ||
| Volume 58, Issue 2, April 2010, Pages 151-162 PDF (3.97 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/vmjg.2010.368189 | ||
| Authors | ||
| Abd El-Malek M1; Ismail A2; Refaie S1 | ||
| 1Food Hygiene Department, Animal Health Research Institute, Assiut Regional Laboratory, Egypt | ||
| 2Food Hygiene Department, Faculty of Veterinary Medicine; Assiut University | ||
| Abstract | ||
| A total number of 75 samples including 25 samples each of luncheon, minced frozen beef and sausage were collected from Assiut city (Upper Egypt) &examined bacteriologically for the presence of E. sakazakii and other Enterobacteriaceae genera& species by using the FDA enrichment procedure. In addition, a polymerase chain reaction (PCR) method for the rapid detection of E. sakazakii was adapted by using a set of primers designed from a region of the 16S ribosomal RNA gene. E. sakazakii was detected in 7 (28%) of sausage, but none of the luncheon and minced frozen beef samples were positive for E. sakazakii. Moreover, one false positive isolate was observed with FDA method. In contrast, PCR was highly sensitive and specific to E. sakazakii. E. cloacae were isolated from all samples, while E. agglomerans was detected only in luncheon samples. Also, 43 isolates related to family Enterobacteriaceae could be isolated including Citrobacter freundii, Escherichia coli, Hafnia alvei, Klebsiella pneumonia, K.oxytoca, K.ozaenae, K. planticola and Serratia marcescens. The public health importance of E. sakazakii and suggested control measures were discussed. | ||
| Keywords | ||
| Enterobacter sakazakii; luncheon; minced beef; sausage; PCR & FDA | ||
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