A, B., A, S., M, B., M, I., M, A., M, A., Α, F. (2011). Preparation of FMD bivalent vaccine A&O by estimation of 146s antigen in the cattle dose and its evaluation by using serological technique and challenge test. test, 59(3), 27-37. doi: 10.21608/vmjg.2011.368228
Bakr A; Saad A; Barghouth M; Ibrahim M; Abd El Aty M; Aly M; Farag Α. "Preparation of FMD bivalent vaccine A&O by estimation of 146s antigen in the cattle dose and its evaluation by using serological technique and challenge test". test, 59, 3, 2011, 27-37. doi: 10.21608/vmjg.2011.368228
A, B., A, S., M, B., M, I., M, A., M, A., Α, F. (2011). 'Preparation of FMD bivalent vaccine A&O by estimation of 146s antigen in the cattle dose and its evaluation by using serological technique and challenge test', test, 59(3), pp. 27-37. doi: 10.21608/vmjg.2011.368228
A, B., A, S., M, B., M, I., M, A., M, A., Α, F. Preparation of FMD bivalent vaccine A&O by estimation of 146s antigen in the cattle dose and its evaluation by using serological technique and challenge test. test, 2011; 59(3): 27-37. doi: 10.21608/vmjg.2011.368228

Preparation of FMD bivalent vaccine A&O by estimation of 146s antigen in the cattle dose and its evaluation by using serological technique and challenge test

Veterinary Medical Journal (Giza)
Volume 59, Issue 3, July 2011, Pages 27-37    PDF (1.72 M)
Document Type: Original Article
DOI: 10.21608/vmjg.2011.368228
Authors
Bakr A* 1; Saad A2; Barghouth M2; Ibrahim M2; Abd El Aty M1; Aly M1; Farag Α1
1Veterinary Serum and Vaccine Research institute, Abbassia, Cairo.
2Central Laboratory for Evaluation of Veterinary Biologics, Abbassia, Cairo.
Abstract
     Foot-and-mouth disease virus (FMDV) serotypes (0/1/3/93) and (A/Egy/2009), grown on BHK-21 clone 13 monolayer cell line, inactivated with Binary Ethylenimine (BEI) and adjuvanted with 30% Alhydragel [AL(OH)3] were used for preparation of bivalent gel adjuvant vaccine currently used in Egypt 146s was measured using sucrose density gradient ultracentrifugation and vaccine dose was estimated as 4.2 µg of 146s viral proteins for each vaccine serotypes. Evaluation and testing of FMD vaccine for safety, sterility and potency were carried out. Cattle protective dose 50% (PD50) was determined. The specific developed neutralizing antibody responses against different doses of bivalent vaccine in correlation with the challenge exposure test for serotype (01/3/93) virus that was done 21 days post vaccination were undertaken. The obtained potency of a vaccine with an overall 50% cattle protective dose (PD50) value was 9.99 (PD50) for serotype (O/1/3/93).
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