Preparation of Hemolysin for Detection of some Viral and Bacterial Antigens | ||
| Veterinary Medical Journal (Giza) | ||
| Volume 59, Issue 3, July 2011, Pages 211-218 PDF (3 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/vmjg.2011.368247 | ||
| Authors | ||
| Manal Mustafa* ; Lamis Tantawy | ||
| Veterinary Serum and Vaccine Research Institute, Abassia, Cairo | ||
| Abstract | ||
| Hemolysin (amboceptor) which is the most important constituent in complement fixation test (CFT) was prepared by inoculation of sheep RBCs S/C in Bosket rabbits in two concentrations (10% and 20%) Blood samples were obtained weekly from inoculated rabbits to measure the titre of induced Hemolysin by CFT. It was found that Hemolysin titer was increased with the number of inoculation till the 7th inoculation then began to decrease with the 8th inoculation. However the titer of Hemolysin obtained from rabbits inoculated with 20% sheep RBCs was higher (1/5800) than that obtained from rabbits inoculated with 10% red cell suspension (1/4500). Similar results were reported to the imported Hemolysin. By using the prepared Hemolysin in CFT for diagnosis of FMD and Brucellosis it gave the same results as that obtained by imported one | ||
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