M, B., Mostafa, H., I, I., Mahmoud, N., M, A., F, Z. (2011). Comparative studies on Application of Enzyme-Linked Immunosorbent Assays and Serum Neutralization Test for Measuring of Bovine Ephemeral Fever Virus Antibodies in Vaccinated Animals. test, 59(3), 19-25. doi: 10.21608/vmjg.2011.368297
Barghouth M; Hanna Mostafa; Ismail I; Nermeen Mahmoud; Abd El-Bakey M; Zaky F. "Comparative studies on Application of Enzyme-Linked Immunosorbent Assays and Serum Neutralization Test for Measuring of Bovine Ephemeral Fever Virus Antibodies in Vaccinated Animals". test, 59, 3, 2011, 19-25. doi: 10.21608/vmjg.2011.368297
M, B., Mostafa, H., I, I., Mahmoud, N., M, A., F, Z. (2011). 'Comparative studies on Application of Enzyme-Linked Immunosorbent Assays and Serum Neutralization Test for Measuring of Bovine Ephemeral Fever Virus Antibodies in Vaccinated Animals', test, 59(3), pp. 19-25. doi: 10.21608/vmjg.2011.368297
M, B., Mostafa, H., I, I., Mahmoud, N., M, A., F, Z. Comparative studies on Application of Enzyme-Linked Immunosorbent Assays and Serum Neutralization Test for Measuring of Bovine Ephemeral Fever Virus Antibodies in Vaccinated Animals. test, 2011; 59(3): 19-25. doi: 10.21608/vmjg.2011.368297

Comparative studies on Application of Enzyme-Linked Immunosorbent Assays and Serum Neutralization Test for Measuring of Bovine Ephemeral Fever Virus Antibodies in Vaccinated Animals

Veterinary Medical Journal (Giza)
Volume 59, Issue 3, July 2011, Pages 19-25    PDF (1.17 M)
Document Type: Original Article
DOI: 10.21608/vmjg.2011.368297
Authors
Barghouth M* 1; Hanna Mostafa1; Ismail I2; Nermeen Mahmoud1; Abd El-Bakey M1; Zaky F1
1Central Laboratory for evaluation of Veterinary Biologics, Abassia, Cairo
2Veterinary Serum and Vaccine Research Institute; Abassia, Cairo
Abstract
 Two models of ELISA were developed for serological detection of Bovine Ephemeral fever (BEF) antibodies in the sera of vaccinated Buffaloes in comparison with serum neutralization test. Blocking ELISA (B. ELISA) was developed and standardized for detection of BEF antibodies. In this test, the binding capacity of rabbit BEF hyper immune sera to BEFV antigen was blocked in the presence of positive BEF vaccinated Buffaloes sera. 
     The sensitivity of both ELISA models was compared with serum neutralization test (SNT) using a total of 160 serum samples obtained from vaccinated Buffaloes where it was found that the sensitivity and specificity of B- ELISA and I-ELISA were (98.4% & not estimated) and (100% & 100%), respectively. These finding suggested that the both ELISA models were considered an inexpensive, rapid and sensitive serological methods that would be suitable for measuring of BEFV antibodies in vaccinated Buffaloes.
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