Genotyping of Staphylococcal Species on the Skin of Patients with Atopic Dermatitis | ||
| Egyptian Journal of Medical Microbiology | ||
| Volume 33, Issue 3, July 2024, Pages 153-163 PDF (597.05 K) | ||
| Document Type: New and original researches in the field of Microbiology. | ||
| DOI: 10.21608/ejmm.2024.303040.1274 | ||
| Authors | ||
| Sara H. Fouad1; Nouran H. Mansour2; Noha M. Mahmoud* 3; Maha A. El Gayyar1 | ||
| 1Dermatology, Andrology and STDs Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt | ||
| 2Dermatology Resident, Belqas General Hospital, Egypt | ||
| 3Medical Microbiology and Immunology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt; Medical Microbiology and Immunology Department, Faculty of Medicine, Horus University, New Damietta, Egypt | ||
| Abstract | ||
| Background: Atopic dermatitis (AD) is a prevalent illness that impacts a diverse range of individuals worldwide. The skin microbiome of AD patients is characterized by increased colonization by Staphylococci and decreased bacterial diversity, which could worsen disease symptoms. Objectives: To recognize staphylococcal species on skin of AD patients and to assess the association of these species with disease severity. Methodology: The study comprised 45 AD patients and 45 healthy controls. Full history, general and dermatological examination were performed to all the participants. Disease severity was calculated using Severity Scoring of Atopic Dermatitis (SCORAD). Microbiological examination was conducted for all skin specimens collected. Molecular identification of the detected staphylococcal species using multiplex Polymerase Chain Reaction (PCR). Results: There was a statistically significant higher growth of Staphylococci among cases than control group (97.8% versus 46.7%, respectively, P value<0.001). Following Genotyping, a statistically significant higher growth of Staphylococcus aureus (S. aureus) and S. hominis were detected among AD cases than control group (P value =0.0005 and 0.05, respectively). There was significant association (p value < 0.001) between severity of AD and genotypic distribution of staphylococcus species. All Patients with Severe AD were colonized with S. aureus. Conclusion: Staphylococcal species were relatively exclusive to AD. Increased frequency of S. aureus and S. hominis were observed in AD skin. These findings may be significant for understanding pathophysiology and severity of AD to outline new treatment options. | ||
| Keywords | ||
| Atopic dermatitis; Staphylococcus aureus; Genotyping; SCORAD | ||
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