Estimation of the proper route and dose for trivalent oily FMD vaccine in sheep | ||
| Veterinary Medical Journal (Giza) | ||
| Volume 60, Issue 3, July 2014, Pages 97-106 PDF (4.84 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/vmjg.2014.368847 | ||
| Authors | ||
| Saad A1; Abd EL Karim S2; Ibrahim M1 | ||
| 1Evaluation of large animal viral vaccine Department, Central Laboratory for Evaluation of Veterinary Biologics (CLEVB) , Cairo, Abbassia, Egypt. | ||
| 2Foot and Mouth Department Veterinary serum and vaccines research institute, Abbassia, Cairo, Egypt. | ||
| Abstract | ||
| This study aims to determine the proper dose and route for vaccination of sheep against foot and mouth disease (FMD), by using trivalent oil FMD vaccine in addition to gaining the highest protective antibody titers. Six groups of sheep (6 sheep / each) were vaccinated with different doses of the trivalent oily FMD vaccine [full (1.5 ml), ½ (0.75ml) and ¼ (0.37ml) doses], S/C or I/M. The experiment included challenged non-vaccinated control group (2 sheep for each used Viral strain). Pre- vaccination blood samples of all groups then weekly blood samples were collected to follow up the level of induced antibody titers by using SNT and ELISA. Four weeks post vaccination all animal groups were challenged by inoculation intradermolingual (IDL) with 10.000 ID50(infective dose fifty) sheep adapted FMD (O & A & SAT2 types) virus and subjected to clinical observation for 8 days. The results showed that the vaccinated animals with a full and ½ dose injected either S/C or I/M were able to withstand the virus infection while animals vaccinated with ¼ dose didn’t. The vaccinated group given full dose through S/C route showed the highest protective antibody titers against all viral strains of the vaccine. | ||
| Keywords | ||
| trivalent FMD vaccine; sheep; dose and route | ||
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