THE EFFECT OF PURIFICATION AND CONCENTRATION OF FMD VIRUS USING POLYETHYLENE GLYCOL ON ITS ANTIGENIC AND IMMUNOGENIC PROPERTIES | ||
| Veterinary Medical Journal (Giza) | ||
| Volume 52, Issue 3, July 2004, Pages 363-386 PDF (2.02 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/vmjg.2004.369298 | ||
| Authors | ||
| M YOUSEF* ; H FAWZY; LILA ELSHAHAWY | ||
| FMD Dept., Vet. Serum and Vaccine Research, Institute, Abbasia, Cairo, Egypt, | ||
| Abstract | ||
| Foot and mouth disease virus was propagated on BHK21 and purified by polyethylene glycol (PEG) precipitation. The antigenic properties of the virus before and after purification were studied by infectivity, complement fixing activity and protein assay tests. Inactivated FMD vaccines were prepared from the purified antigen and the crude one. The potency of both vaccines was tested in guinea pigs. The results revealed that the process of PEG precipitation of the virus will improve the quality of antigen used in vaccine formulation to produce a good quality FMD vaccine. | ||
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