TAHA, M., ELIAN, K., Mahmoud, G., HASSAN, K., MOHAMMED, F. (2002). STANDARDIZATION OF BOTH RIFT VALLEY FEVER CELL LYSATE AND SUPERNATANT ANTIGENS USED IN ELISA COMPARING WITH SERUM NEUTRALIZATION TEST AND AGAR GEL PRECIPITATION TEST. test, 50(4), 987-992. doi: 10.21608/vmjg.2002.369377
M TAHA; KHAIRAT ELIAN; GIHAN Mahmoud; K HASSAN; FATMA MOHAMMED. "STANDARDIZATION OF BOTH RIFT VALLEY FEVER CELL LYSATE AND SUPERNATANT ANTIGENS USED IN ELISA COMPARING WITH SERUM NEUTRALIZATION TEST AND AGAR GEL PRECIPITATION TEST". test, 50, 4, 2002, 987-992. doi: 10.21608/vmjg.2002.369377
TAHA, M., ELIAN, K., Mahmoud, G., HASSAN, K., MOHAMMED, F. (2002). 'STANDARDIZATION OF BOTH RIFT VALLEY FEVER CELL LYSATE AND SUPERNATANT ANTIGENS USED IN ELISA COMPARING WITH SERUM NEUTRALIZATION TEST AND AGAR GEL PRECIPITATION TEST', test, 50(4), pp. 987-992. doi: 10.21608/vmjg.2002.369377
TAHA, M., ELIAN, K., Mahmoud, G., HASSAN, K., MOHAMMED, F. STANDARDIZATION OF BOTH RIFT VALLEY FEVER CELL LYSATE AND SUPERNATANT ANTIGENS USED IN ELISA COMPARING WITH SERUM NEUTRALIZATION TEST AND AGAR GEL PRECIPITATION TEST. test, 2002; 50(4): 987-992. doi: 10.21608/vmjg.2002.369377

STANDARDIZATION OF BOTH RIFT VALLEY FEVER CELL LYSATE AND SUPERNATANT ANTIGENS USED IN ELISA COMPARING WITH SERUM NEUTRALIZATION TEST AND AGAR GEL PRECIPITATION TEST

Veterinary Medical Journal (Giza)
Volume 50, Issue 4, October 2002, Pages 987-992    PDF (1.53 M)
Document Type: Original Article
DOI: 10.21608/vmjg.2002.369377
Authors
M TAHA* ; KHAIRAT ELIAN; GIHAN Mahmoud; K HASSAN; FATMA MOHAMMED
Veterinary Serum and Vaccine Research Institute, Abbassia, Cairo
Abstract
To compare between different serological tests and in trial to standardize both RVF cell lysate and cell supernatant antigens, 1377 serum sam- ples collected from different Egyptian Governorates from different species (sheep, goat, cattle and buffalo) were tested for the presence of IgG and IgM percent using ELISA. The result ranged from 61.06 63.1 for IgG and 13.73 15.3 for IgM. ELISA, NI and AGPT results were higher from 1:4 months than 1 9 months. At the same time ELISA gave higher figure of sensitivity than NI and AGPT.
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