Abdelmoneim, S., Madbouly, H., Gelb, J., Ladman, B. (2002). Isolation and identification of Egypt/Beni-Seuf/01: a novel genotype of infectious bronchitis virus. test, 50(4), 1056-1078. doi: 10.21608/vmjg.2002.369387
S Abdelmoneim; H Madbouly; J Gelb; B Ladman. "Isolation and identification of Egypt/Beni-Seuf/01: a novel genotype of infectious bronchitis virus". test, 50, 4, 2002, 1056-1078. doi: 10.21608/vmjg.2002.369387
Abdelmoneim, S., Madbouly, H., Gelb, J., Ladman, B. (2002). 'Isolation and identification of Egypt/Beni-Seuf/01: a novel genotype of infectious bronchitis virus', test, 50(4), pp. 1056-1078. doi: 10.21608/vmjg.2002.369387
Abdelmoneim, S., Madbouly, H., Gelb, J., Ladman, B. Isolation and identification of Egypt/Beni-Seuf/01: a novel genotype of infectious bronchitis virus. test, 2002; 50(4): 1056-1078. doi: 10.21608/vmjg.2002.369387

Isolation and identification of Egypt/Beni-Seuf/01: a novel genotype of infectious bronchitis virus

Veterinary Medical Journal (Giza)
Volume 50, Issue 4, October 2002, Pages 1056-1078    PDF (3.69 M)
Document Type: Original Article
DOI: 10.21608/vmjg.2002.369387
Authors
S Abdelmoneim* 1; H Madbouly1; J Gelb2; B Ladman2
1Department of virology, Faculty of Veterinary Medicine, Cairo University Beni-Seuf branch
2Department of Animal and Food Science, University of Delaware
Abstract
 
Infectious bronchitis virus (IBV) strain Egypt/Beni-Seuf/01 was isolated from 38-day-old broiler chickens suffering from respiratory and renal disease in the Beni-Seuf governorate of upper Egypt in 1998. The strain was identified using electron microscopy (EM), agar gel precipitation test (AGPT), and reverse transcriptase polymerase chain reaction (RT-PCR). SI gene cycle sequencing of the RT-PCR product was used to determine the genotype of the strain. Three weeks old commercial ‘chickens were vaccinated with the IBV vaccine (H 120) and the protection level against challenge Egypt/Beni-Seuf/01 was evaluated via viral isolation attempts from tracheal scraping suspensions as well as tracheal histopathology at 4 days post-challenge. Kidney histopathology at 4 days post-challenge was examined. AGPT on the chorioallantoic membrane(CAM) homogenate of each passage using rabbit anti-IBV polyclonal serum showed positive results with all egg passages (Ist to 7th ). E/M examination revealed pleomorphic or round virions with widely spaced surface projections and 160nm average diameter. Alignment of the S1 sequence of Egypt\Beni-Seuf/01 with 22 published IBV strains demonstrated that the newly deposited sequence of Israel/720/99 is closely related to the Egyptian isolate. Vaccination of broiler-type chickens with the live H120 strain offered only 20% protection against challenges with the Egyptian strain. Kidney histo-pathological lesions indicated that Egypt\Beni-Seuf/Ol is a nephrogenic IBV. Results of SI sequencing and the vaccination trial indicate that the Egypt\Beni-Seuf/01 strain is a novel genotype for which H120 vaccination does not provide pro-infection.
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