Karam, A., Azzam, A., Ebrahim, R., Mostafa, A. (2015). Antimicrobial susceptibility of coagulase-negative staphylococci isolated from bovine mastitis. test, 61(1), 23-28. doi: 10.21608/vmjg.2015.369478
Alawer Karam; Alkordi Azzam; Rifai Ebrahim; A. Shalaby Mostafa. "Antimicrobial susceptibility of coagulase-negative staphylococci isolated from bovine mastitis". test, 61, 1, 2015, 23-28. doi: 10.21608/vmjg.2015.369478
Karam, A., Azzam, A., Ebrahim, R., Mostafa, A. (2015). 'Antimicrobial susceptibility of coagulase-negative staphylococci isolated from bovine mastitis', test, 61(1), pp. 23-28. doi: 10.21608/vmjg.2015.369478
Karam, A., Azzam, A., Ebrahim, R., Mostafa, A. Antimicrobial susceptibility of coagulase-negative staphylococci isolated from bovine mastitis. test, 2015; 61(1): 23-28. doi: 10.21608/vmjg.2015.369478

Antimicrobial susceptibility of coagulase-negative staphylococci isolated from bovine mastitis

Veterinary Medical Journal (Giza)
Volume 61, Issue 1, January 2015, Pages 23-28    PDF (3.12 M)
DOI: 10.21608/vmjg.2015.369478
Authors
Alawer Karam* 1; Alkordi Azzam1; Rifai Ebrahim1; A. Shalaby Mostafa2
1Department of Microbiology, Faculty of Veterinary Medicine, Cairo University
2Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University
Abstract
The present study aimed to determine the in vivo susceptibility of coagulase-negative staphylococci (CNS) isolated from milk samples in cases of clinical and subclinical bovine mastitis to some antimicrobial drugs.  The antibacterial sensitivity was tested using the disk diffusion method and performed according to the National Committee for Clinical and Laboratory Standards (NCCLS) guidelines using Mueller-Hinton agar. A total of 180 milk samples from apparently healthy cows and 130 milk samples from cows suffering from bovine mastitis were collected. Subclinical mastitis was detected using the California test and bacterial isolation and identification of CNS isolates were carried out depending on colony morphology and biochemical tests. The results showed that a total of 368 (45.77%) isolates of CNS were obtained from clinical and subclinical mastitis. Coagulase-negative staphylococci were dominant in 366 (54.3) isolates from subclinical mastitis and only 2 isolates from clinical mastitis. The highest number of CNS isolates was susceptible to chloramphenicol (84.78%), followed by tetracycline (80.43%) and kanamycin (74.46%). Whereas 40.76 % of CNS isolates were resistant to penicillin. Before starting the treatment of mastitis, the antibiogram for different antibacterial agents is necessary to attain the maximum efficacy of the antibiotic used and to prevent the emergence of bacterial resistance.
Keywords
Antimicrobial susceptibility; Coagulase-negative staphylococci; Bovine mastitis
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