Utility of Redox Reactions to Determine Some Cephalosporins Spectrophotometrically | ||
| Journal of Basic and Environmental Sciences | ||
| Volume 4, Issue 4, October 2017, Pages 356-361 PDF (297.66 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jbes.2017.369708 | ||
| Authors | ||
| Mostafa Y. Nassar; Alaa S. Amin; M.M. Moustafa; Ahmed.A. Khalial | ||
| Chemistry Department, Faculty of Science, Benha University, Benha, Egypt | ||
| Abstract | ||
| A simple, accurate and sensitive spectrophotometric method for the determination of cefadroxil (CXL), cefaclor (CLR) and cefatoxime (CTXM) in pure, dosage forms, and in the presence of its oxidative degradates is described. The method is based on the oxidation of the studied drug using cerium(IV) ammonium sulphate and subsequent determination of the unreacted Ce(IV) by measuring the decrease in absorbance of rhodamine B (RhB) at a suitable λmax= 540, 541 and 542 nm for CXL, CLR and CTXM, respectively. Regression analysis of Beer/Lambert plots showed good correlation in the concentration ranges: 1.0-10, 1.0-12 and 2.0-8.0 µg ml−1 for CXL, CLR and CTXM, respectively. The apparent molar absorptivity, Sandell sensitivity, detection and quantitation limits were calculated. For more accurate results, Ringbom optimum concentration ranges were 3.0-8.0, 3.0-9.0 and 4.0-7.0 µg ml−1 , respectively. The obtained results are compared statistically using student's t-test and variance ratio F-test with the official methods. Analyzing pure and dosage forms containing CXL, CLR and CTXM tested the validity of the proposed method. The relative standard deviations were ≤ 1.38 with recoveries 98.2-101.2%. | ||
| Keywords | ||
| Cephalosporins determination; Redox reaction; Spectrophotometry; Dosage forms | ||
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