SHAWKY, M., SABER, M., ABDEL RAHMAN, A., EL-SONOUSI, A. (1999). CLONING AND EXPRESSION OF 3CD GENES OF FMDV. test, 47(3), 289-294. doi: 10.21608/vmjg.1999.370851
M SHAWKY; M SABER; A ABDEL RAHMAN; A EL-SONOUSI. "CLONING AND EXPRESSION OF 3CD GENES OF FMDV". test, 47, 3, 1999, 289-294. doi: 10.21608/vmjg.1999.370851
SHAWKY, M., SABER, M., ABDEL RAHMAN, A., EL-SONOUSI, A. (1999). 'CLONING AND EXPRESSION OF 3CD GENES OF FMDV', test, 47(3), pp. 289-294. doi: 10.21608/vmjg.1999.370851
SHAWKY, M., SABER, M., ABDEL RAHMAN, A., EL-SONOUSI, A. CLONING AND EXPRESSION OF 3CD GENES OF FMDV. test, 1999; 47(3): 289-294. doi: 10.21608/vmjg.1999.370851

CLONING AND EXPRESSION OF 3CD GENES OF FMDV

Veterinary Medical Journal (Giza)
Volume 47, Issue 3, July 1999, Pages 289-294    PDF (1.79 M)
Document Type: Original Article
DOI: 10.21608/vmjg.1999.370851
Authors
M SHAWKY1; M SABER2; A ABDEL RAHMAN1; A EL-SONOUSI1
1FMD Department, Veterinary Serum and Vaccine Research Institute, Abbassia, Cairo.
2Faculty of Veterinary Medicine, Cairo University.
Abstract
RT-PCR amplification of VIA (3D) gene and protease (3C) using its primers. Legation of the PCR product with the cloning vector (using pET system). Expression of the re- combinant plasmid in E. coli expression host. Use of the expressed VIA protein in diagnosis of cattle infected with any of the 7 serotypes of FMD virus with the help of ELISA.
 
 
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