MAHMOUD, M., SIAM, M. (1994). COMPARATIVE ANALYSIS OF ASCARIDIAL PARASITIC CRUDE ANTIGEN BY SDS-PAGE AND UV ABSORBANCE TECHNIQUES. test, 42(1), 41-45. doi: 10.21608/vmjg.1994.372013
M MAHMOUD; M SIAM. "COMPARATIVE ANALYSIS OF ASCARIDIAL PARASITIC CRUDE ANTIGEN BY SDS-PAGE AND UV ABSORBANCE TECHNIQUES". test, 42, 1, 1994, 41-45. doi: 10.21608/vmjg.1994.372013
MAHMOUD, M., SIAM, M. (1994). 'COMPARATIVE ANALYSIS OF ASCARIDIAL PARASITIC CRUDE ANTIGEN BY SDS-PAGE AND UV ABSORBANCE TECHNIQUES', test, 42(1), pp. 41-45. doi: 10.21608/vmjg.1994.372013
MAHMOUD, M., SIAM, M. COMPARATIVE ANALYSIS OF ASCARIDIAL PARASITIC CRUDE ANTIGEN BY SDS-PAGE AND UV ABSORBANCE TECHNIQUES. test, 1994; 42(1): 41-45. doi: 10.21608/vmjg.1994.372013

COMPARATIVE ANALYSIS OF ASCARIDIAL PARASITIC CRUDE ANTIGEN BY SDS-PAGE AND UV ABSORBANCE TECHNIQUES

Veterinary Medical Journal (Giza)
Volume 42, Issue 1, January 1994, Pages 41-45    PDF (1.54 M)
Document Type: Original Article
DOI: 10.21608/vmjg.1994.372013
Authors
M MAHMOUD* 1; M SIAM2
1Animal Health Research Inst., Dokki, Giza, Egypt.
2Fac. of Vet. Med., Cairo Unviersity, Egypt.
Abstract
 Ascaridial nematodes are among the well-known parasites infesting fish, birds and mammals. Crude antigen was extracted from Amplicaecum larvae (AL3), Ascaridia galli (adult worm-AG) and Neoascaris vitulorum (adult worm-AV). The charactcrization of these parasitic nematodes revealed relative homologous polypeptide chains (MW 25.5 KDa) common to the three parasitic species, whereas the UV absorbance scan mode wave length (WL 200/550 nm) as related to the absorbance (AB) value zero-2 recorded an indicative frequency curve. The protein concentration in Amplicaecum, Ascaridia and Neoascaris figured up to 5.47 g%, 4.93 g% and 2.20 g%, respectively.
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