SEROEPIDEMIOLOGICAL AND VIROLOGICAL STUDIES ON VIRUS-INDUCED TUMORS IN CHICKENS IN EGYPT* | ||
| Veterinary Medical Journal (Giza) | ||
| Volume 49, Issue 2, April 2001, Pages 237-247 PDF (3.14 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/vmjg.2001.372110 | ||
| Authors | ||
| AFAF A AMIN* 1; MONA M ALY1; ASSIA EL-SAWY1; N TANIOUS1; A KHAFAGY1; A AHMED2 | ||
| 1Animal Health Research Institute, Dokki, Giza. | ||
| 2Faculty of Veterinary medicine, Univ. of Alexandria | ||
| Abstract | ||
| Extensive epidemiological studies were conducted over four years to investigate the real cause And prevalence of virus-induced tumors among Commercial meat-and egg-type chicken breeds, as Well as native varieties of different age-groups And localities. Criteria used to establish an etiological diagnosis in problem flocks included, besides flock history, gross, histopathological and Cytological examinations, virus isolation in chicken embryo fibroblast cultures (Line O) and identification by enzyme-linked-immunosorbent assay (ELISA) and/or indirect immunofluorescence (IFA), antigen detection in thin tissue sections by IFA, and reticuloendothelisosis virus (REV) pro-viral DNA detection in blood or tumor tissue by Polymerase chain reaction (PCR). Moreover, Chicken flocks were examined for viral antigen and/or antibody in plasma, egg albumen or serum By ELIA. The results achieved indicated that Marck’s disease virus (MDV), lymphoid leukosis virus (LLV), and REV were the common causes of neoplasms as single or mixed infections with variable Incidence among the flocks. The sources of infection in the investigated flocks is discussed in the light of the obtained results. | ||
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