Centella Asiatica improves Aluminum Chloride-induced toxicity in the rat liver and kidney by reducing oxidative stress and DNA damage | ||
| Egyptian Journal of Histology | ||
| Articles in Press, Accepted Manuscript, Available Online from 06 August 2024 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejh.2024.296103.2085 | ||
| Authors | ||
| Shyimaa Hassan* 1; Azza M Gawish2; Hanan R Hamed2; Shaymaa M Eissa1 | ||
| 1Zoology Department, Faculty of science, Cairo Univeristy | ||
| 2Zoology Department, Faculty of science, Cairo University | ||
| Abstract | ||
| Background: Aluminum (Al) is a harmful element to human beings and animals widely used worldwide. It has been associated with the incidence of several diseases. Centella Asiatica (CA) has been utilized as a natural antioxidant, hepatoprotective, and anti-inflammatory agent. Aim of the Work: the current study aims to assess the safeguard function of CA extract against aluminum chloride (AlCl3) provoked hepatotoxicity and nephrotoxicity in rats. Material and Methods: Forty-eight rats were distributed into 8 groups. Control group received dist. water and Al-group was given AlCl3 (100 mg/kg); groups (3, 4 &5) were given various dosages of CA treatment (200, 400, and 600 mg/kg); and groups (6, 7 &8) were treated with both CA (200, 400, and 600 mg/kg respectively) and AlCl3 (100 mg/kg) orally for five weeks. Oxidative stress biomarkers Malondialdehyde (MDA), Superoxide dismutase (SOD), and Glutathione (GSH) were assessed, the comet assay was used to assess the level of DNA damage in liver and kidney tissues, additionally, histopathological examinations were performed. Results: CA treatment effectively eliminated free radicals and restored oxidant/antioxidant balance in the liver and kidneys. It decreased MDA levels and increased the activity of GSH and SOD. CA treatment also decreased DNA damage induced by Al. Histopathological studies indicated that CA extract improved the architecture of the liver and decreased the infiltration of inflammatory cells caused by Al. Conclusion: Centella Asiatica can restore tissue homeostasis by increasing antioxidant enzymes, eliminating ROS, and serving as a protective agent for DNA, thus safeguarding liver and kidney tissues from aluminum-induced toxicity | ||
| Keywords | ||
| CA extract; inflammation; AlCl3; nephrotoxicity; hepatotoxicity | ||
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